Source 1review2014Cold Spring Harbor Protocols
Toolkit/conditional gene mutation
conditional gene mutation
Also known as: conditional gene knockout, conditional mutations
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Conditional gene mutation is a genetic engineering method for creating gene alterations that are activated in spatially restricted and/or temporally restricted ways. In mice, these conditional mutations enable controlled genetic perturbation for experimental studies and disease modeling.
Usefulness & Problems
Why this is useful
This method is useful because it allows gene alterations to be introduced only in selected tissues or at selected times, rather than constitutively. Mouse strains carrying conditional mutations are reported to be valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Problem solved
Conditional gene mutation addresses the problem of needing controlled gene perturbation instead of unrestricted mutation throughout an organism or across development. The cited literature specifically supports spatial and temporal restriction of gene alteration in mice.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Mechanisms
spatially restricted gene mutationspatially restricted gene mutationtemporally restricted gene mutationtemporally restricted gene mutationTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: builder
The evidence indicates application in mice and refers generally to new technology enabling conditional mutations. No specific construct architecture, recombinase, delivery method, promoter strategy, or breeding scheme is described in the supplied material.
The supplied evidence does not specify particular recombination systems, target loci, efficiencies, or failure modes. It also does not provide comparative performance data, organismal scope beyond mice, or independent validation details.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug conditional-gene-mutation
New technology has made it possible to generate conditional mutations that can be introduced in a spatially and/or temporally restricted manner.
Source:
advantage of conditional mutationsupports
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Source:
disease modeling valuesupports
Mouse strains carrying conditional mutations are valuable experimental models for studying human diseases and for developing prevention and treatment strategies.
Source:
limitation of germline mutationsupports
Traditional transgenic and knockout mice can be limited for disease studies because germline-passed mutations may profoundly affect development and impede study of adult disease phenotypes.
Source:
model system suitabilitysupports
The laboratory mouse is presented as an ideal model organism for studying disease because it is physiologically similar to humans and its genome is readily manipulated.
Source:
Comparisons
Source-backed strengths
A key strength is the ability to restrict gene mutation by location and/or time, enabling more controlled experimental design. The available evidence also supports its value in generating mouse models relevant to human disease research and therapeutic strategy development.
Source:
Conditional mutations can be introduced in a spatially and/or temporally restricted manner.
Ranked Citations
- 1.