Workflows
Design–Build–Test–Learn loop archetypes with step-level timing, testing order, and rationale for how mechanism and technique choices come together.
Screening Funnel
Higher-level screening gates show how the workflow narrows candidate space before the detailed step-by-step timeline.
broad first-pass screen
Broad ScreenCell-Free8 candidate designs -> 2 candidate designs
measurable signal in a rapid assay with acceptable background
Advance when: clear stimulated-versus-baseline separation in the first-pass assay
Bottleneck risk: DNA acquisition still dominates the calendar even in a fast screen
Enriches for: time to first signal, basic functional activity
Guards against: spending cloning time on clearly weak designs, early host-specific confounding
go or no-go decision
Decision GateCell-Based2 shortlisted designs -> 1 shortlisted designs
whether the first-pass hit is strong enough to justify a slower host-context loop
Advance when: only carry forward designs that justify a more realistic validation loop
Preserves downstream: downstream build capacity, host-context follow-up bandwidth
Loop Map
A schema-driven DBTL view that groups ordered workflow steps into design, build, test, and learn phases, then shows how the workflow loops back for the next iteration.
Design
Define the construct, library, or study plan.
Build
Make or prepare the material you will evaluate.
Test
Run the assay or selection that produces signal.
Learn
Interpret the result and decide what to do next.
Phase Breakdown
A high-level view of how the workflow collapses into concrete lab or analysis steps.
Design
1 stepDefine the construct, library, or study plan.
Build
1 stepMake or prepare the material you will evaluate.
Test
1 stepRun the assay or selection that produces signal.
Learn
1 stepInterpret the result and decide what to do next.