Source 1primary paper2024
Toolkit/DHFR/LOV2 fusion
DHFR/LOV2 fusion
Also known as: engineered photoswitch, light-regulated Dihydrofolate Reductase (DHFR) enzyme
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The DHFR/LOV2 fusion is an engineered photoswitch in which the LOV2 light-sensing module was used to create a light-regulated dihydrofolate reductase (DHFR) enzyme. Source evidence indicates that light activation modulates DHFR catalysis through allosteric effects associated with local disorder and altered transition-state thermodynamics.
Usefulness & Problems
Why this is useful
This tool is useful for studying how light-responsive allostery can be coupled to enzyme catalysis in a defined fusion protein. The available evidence specifically supports its value as a model system for dissecting relationships among photosensory input, local disorder, thermal stability, and catalytic output.
Problem solved
It addresses the problem of engineering an enzyme whose catalytic behavior can be regulated by light through an appended sensory domain. The cited work also uses this system to probe how allosteric tuning mutations reshape the tradeoff between activity and stability.
Problem links
Need conditional recombination or state switching
DerivedThe DHFR/LOV2 fusion is an engineered photoswitch in which the LOV2 light-sensing module was used to create a light-regulated dihydrofolate reductase (DHFR) enzyme. Source evidence supports that light activation alters DHFR catalysis through allosteric effects linked to local disorder and changes in transition-state thermodynamics.
Need precise spatiotemporal control with light input
DerivedThe DHFR/LOV2 fusion is an engineered photoswitch in which the LOV2 light-sensing module was used to create a light-regulated dihydrofolate reductase (DHFR) enzyme. Source evidence supports that light activation alters DHFR catalysis through allosteric effects linked to local disorder and changes in transition-state thermodynamics.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
allosteric switchingallosteric switchingenthalpy-entropy compensation in transition-state regulationenthalpy-entropy compensation in transition-state regulationlight-dependent photosensory regulationlight-dependent photosensory regulationlocal disorder-mediated catalytic modulationlocal disorder-mediated catalytic modulationTechniques
No technique tags yet.
Target processes
recombinationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: actuatoroperating role: sensorswitch architecture: multi componentswitch architecture: uncaging
The construct is described as a fusion in which LOV2 was used to create a light-regulated DHFR enzyme, supporting domain-fusion-based implementation. The provided evidence does not report expression system, chromophore requirements, linker design, or delivery considerations.
Evidence is currently limited to a single cited 2024 study and a small set of mechanistic claims. The supplied evidence does not specify quantitative performance metrics, illumination wavelengths, host organism, construct architecture, or validation in applications beyond the engineered enzyme context.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Many allostery-tuning mutations showed a negative correlation between light-induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many of the allostery tuning mutations showed a negative correlation between the light induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many allostery-tuning mutations showed a negative correlation between light-induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many of the allostery tuning mutations showed a negative correlation between the light induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many allostery-tuning mutations showed a negative correlation between light-induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many of the allostery tuning mutations showed a negative correlation between the light induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many allostery-tuning mutations showed a negative correlation between light-induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many of the allostery tuning mutations showed a negative correlation between the light induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many allostery-tuning mutations showed a negative correlation between light-induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many of the allostery tuning mutations showed a negative correlation between the light induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many allostery-tuning mutations showed a negative correlation between light-induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many of the allostery tuning mutations showed a negative correlation between the light induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many allostery-tuning mutations showed a negative correlation between light-induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many of the allostery tuning mutations showed a negative correlation between the light induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many allostery-tuning mutations showed a negative correlation between light-induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many of the allostery tuning mutations showed a negative correlation between the light induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many allostery-tuning mutations showed a negative correlation between light-induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many of the allostery tuning mutations showed a negative correlation between the light induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many allostery-tuning mutations showed a negative correlation between light-induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many of the allostery tuning mutations showed a negative correlation between the light induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many allostery-tuning mutations showed a negative correlation between light-induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many of the allostery tuning mutations showed a negative correlation between the light induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many allostery-tuning mutations showed a negative correlation between light-induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many of the allostery tuning mutations showed a negative correlation between the light induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many allostery-tuning mutations showed a negative correlation between light-induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many of the allostery tuning mutations showed a negative correlation between the light induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many allostery-tuning mutations showed a negative correlation between light-induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many of the allostery tuning mutations showed a negative correlation between the light induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many allostery-tuning mutations showed a negative correlation between light-induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many of the allostery tuning mutations showed a negative correlation between the light induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many allostery-tuning mutations showed a negative correlation between light-induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many of the allostery tuning mutations showed a negative correlation between the light induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many allostery-tuning mutations showed a negative correlation between light-induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many of the allostery tuning mutations showed a negative correlation between the light induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Local disorder is associated with enhanced catalysis in the engineered photoswitch.
Local disorder is associated with enhanced catalysis in an engineered photoswitch
Local disorder is associated with enhanced catalysis in the engineered photoswitch.
Local disorder is associated with enhanced catalysis in an engineered photoswitch
Local disorder is associated with enhanced catalysis in the engineered photoswitch.
Local disorder is associated with enhanced catalysis in an engineered photoswitch
Local disorder is associated with enhanced catalysis in the engineered photoswitch.
Local disorder is associated with enhanced catalysis in an engineered photoswitch
Local disorder is associated with enhanced catalysis in the engineered photoswitch.
Local disorder is associated with enhanced catalysis in an engineered photoswitch
Local disorder is associated with enhanced catalysis in the engineered photoswitch.
Local disorder is associated with enhanced catalysis in an engineered photoswitch
Local disorder is associated with enhanced catalysis in the engineered photoswitch.
Local disorder is associated with enhanced catalysis in an engineered photoswitch
Local disorder is associated with enhanced catalysis in the engineered photoswitch.
Local disorder is associated with enhanced catalysis in an engineered photoswitch
Local disorder is associated with enhanced catalysis in the engineered photoswitch.
Local disorder is associated with enhanced catalysis in an engineered photoswitch
Local disorder is associated with enhanced catalysis in the engineered photoswitch.
Local disorder is associated with enhanced catalysis in an engineered photoswitch
Local disorder is associated with enhanced catalysis in the engineered photoswitch.
Local disorder is associated with enhanced catalysis in an engineered photoswitch
Local disorder is associated with enhanced catalysis in the engineered photoswitch.
Local disorder is associated with enhanced catalysis in an engineered photoswitch
Local disorder is associated with enhanced catalysis in the engineered photoswitch.
Local disorder is associated with enhanced catalysis in an engineered photoswitch
Local disorder is associated with enhanced catalysis in the engineered photoswitch.
Local disorder is associated with enhanced catalysis in an engineered photoswitch
Local disorder is associated with enhanced catalysis in the engineered photoswitch.
Local disorder is associated with enhanced catalysis in an engineered photoswitch
Local disorder is associated with enhanced catalysis in the engineered photoswitch.
Local disorder is associated with enhanced catalysis in an engineered photoswitch
Local disorder is associated with enhanced catalysis in the engineered photoswitch.
Local disorder is associated with enhanced catalysis in an engineered photoswitch
The energetic effect of light activation on transition state free energy was composed of a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on the transition state free energy was composed of two opposing forces: a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on transition state free energy was composed of a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on the transition state free energy was composed of two opposing forces: a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on transition state free energy was composed of a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on the transition state free energy was composed of two opposing forces: a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on transition state free energy was composed of a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on the transition state free energy was composed of two opposing forces: a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on transition state free energy was composed of a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on the transition state free energy was composed of two opposing forces: a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on transition state free energy was composed of a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on the transition state free energy was composed of two opposing forces: a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on transition state free energy was composed of a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on the transition state free energy was composed of two opposing forces: a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on transition state free energy was composed of a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on the transition state free energy was composed of two opposing forces: a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on transition state free energy was composed of a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on the transition state free energy was composed of two opposing forces: a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on transition state free energy was composed of a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on the transition state free energy was composed of two opposing forces: a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on transition state free energy was composed of a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on the transition state free energy was composed of two opposing forces: a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on transition state free energy was composed of a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on the transition state free energy was composed of two opposing forces: a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on transition state free energy was composed of a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on the transition state free energy was composed of two opposing forces: a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on transition state free energy was composed of a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on the transition state free energy was composed of two opposing forces: a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on transition state free energy was composed of a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on the transition state free energy was composed of two opposing forces: a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on transition state free energy was composed of a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on the transition state free energy was composed of two opposing forces: a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on transition state free energy was composed of a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on the transition state free energy was composed of two opposing forces: a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
Allostery-tuning mutations in DHFR modulated the enthalpy-entropy tradeoff by either accentuating the enthalpic benefit or minimizing the entropic penalty, but not both.
Allostery-tuning mutations in DHFR acted through this tradeoff, either accentuating the enthalpic benefit or minimizing the entropic penalty but never improving both.
Allostery-tuning mutations in DHFR modulated the enthalpy-entropy tradeoff by either accentuating the enthalpic benefit or minimizing the entropic penalty, but not both.
Allostery-tuning mutations in DHFR acted through this tradeoff, either accentuating the enthalpic benefit or minimizing the entropic penalty but never improving both.
Allostery-tuning mutations in DHFR modulated the enthalpy-entropy tradeoff by either accentuating the enthalpic benefit or minimizing the entropic penalty, but not both.
Allostery-tuning mutations in DHFR acted through this tradeoff, either accentuating the enthalpic benefit or minimizing the entropic penalty but never improving both.
Allostery-tuning mutations in DHFR modulated the enthalpy-entropy tradeoff by either accentuating the enthalpic benefit or minimizing the entropic penalty, but not both.
Allostery-tuning mutations in DHFR acted through this tradeoff, either accentuating the enthalpic benefit or minimizing the entropic penalty but never improving both.
Allostery-tuning mutations in DHFR modulated the enthalpy-entropy tradeoff by either accentuating the enthalpic benefit or minimizing the entropic penalty, but not both.
Allostery-tuning mutations in DHFR acted through this tradeoff, either accentuating the enthalpic benefit or minimizing the entropic penalty but never improving both.
Allostery-tuning mutations in DHFR modulated the enthalpy-entropy tradeoff by either accentuating the enthalpic benefit or minimizing the entropic penalty, but not both.
Allostery-tuning mutations in DHFR acted through this tradeoff, either accentuating the enthalpic benefit or minimizing the entropic penalty but never improving both.
Allostery-tuning mutations in DHFR modulated the enthalpy-entropy tradeoff by either accentuating the enthalpic benefit or minimizing the entropic penalty, but not both.
Allostery-tuning mutations in DHFR acted through this tradeoff, either accentuating the enthalpic benefit or minimizing the entropic penalty but never improving both.
Allostery-tuning mutations in DHFR modulated the enthalpy-entropy tradeoff by either accentuating the enthalpic benefit or minimizing the entropic penalty, but not both.
Allostery-tuning mutations in DHFR acted through this tradeoff, either accentuating the enthalpic benefit or minimizing the entropic penalty but never improving both.
Allostery-tuning mutations in DHFR modulated the enthalpy-entropy tradeoff by either accentuating the enthalpic benefit or minimizing the entropic penalty, but not both.
Allostery-tuning mutations in DHFR acted through this tradeoff, either accentuating the enthalpic benefit or minimizing the entropic penalty but never improving both.
Allostery-tuning mutations in DHFR modulated the enthalpy-entropy tradeoff by either accentuating the enthalpic benefit or minimizing the entropic penalty, but not both.
Allostery-tuning mutations in DHFR acted through this tradeoff, either accentuating the enthalpic benefit or minimizing the entropic penalty but never improving both.
Allostery-tuning mutations in DHFR modulated the enthalpy-entropy tradeoff by either accentuating the enthalpic benefit or minimizing the entropic penalty, but not both.
Allostery-tuning mutations in DHFR acted through this tradeoff, either accentuating the enthalpic benefit or minimizing the entropic penalty but never improving both.
Allostery-tuning mutations in DHFR modulated the enthalpy-entropy tradeoff by either accentuating the enthalpic benefit or minimizing the entropic penalty, but not both.
Allostery-tuning mutations in DHFR acted through this tradeoff, either accentuating the enthalpic benefit or minimizing the entropic penalty but never improving both.
Allostery-tuning mutations in DHFR modulated the enthalpy-entropy tradeoff by either accentuating the enthalpic benefit or minimizing the entropic penalty, but not both.
Allostery-tuning mutations in DHFR acted through this tradeoff, either accentuating the enthalpic benefit or minimizing the entropic penalty but never improving both.
Allostery-tuning mutations in DHFR modulated the enthalpy-entropy tradeoff by either accentuating the enthalpic benefit or minimizing the entropic penalty, but not both.
Allostery-tuning mutations in DHFR acted through this tradeoff, either accentuating the enthalpic benefit or minimizing the entropic penalty but never improving both.
Allostery-tuning mutations in DHFR modulated the enthalpy-entropy tradeoff by either accentuating the enthalpic benefit or minimizing the entropic penalty, but not both.
Allostery-tuning mutations in DHFR acted through this tradeoff, either accentuating the enthalpic benefit or minimizing the entropic penalty but never improving both.
Allostery-tuning mutations in DHFR modulated the enthalpy-entropy tradeoff by either accentuating the enthalpic benefit or minimizing the entropic penalty, but not both.
Allostery-tuning mutations in DHFR acted through this tradeoff, either accentuating the enthalpic benefit or minimizing the entropic penalty but never improving both.
Allostery-tuning mutations in DHFR modulated the enthalpy-entropy tradeoff by either accentuating the enthalpic benefit or minimizing the entropic penalty, but not both.
Allostery-tuning mutations in DHFR acted through this tradeoff, either accentuating the enthalpic benefit or minimizing the entropic penalty but never improving both.
LOV2 photoactivation lowered the catalytic transition free energy of the lit state relative to the dark state in the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (2) lowered the catalytic transition free energy of the lit state relative to the dark state.
LOV2 photoactivation lowered the catalytic transition free energy of the lit state relative to the dark state in the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (2) lowered the catalytic transition free energy of the lit state relative to the dark state.
LOV2 photoactivation lowered the catalytic transition free energy of the lit state relative to the dark state in the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (2) lowered the catalytic transition free energy of the lit state relative to the dark state.
LOV2 photoactivation lowered the catalytic transition free energy of the lit state relative to the dark state in the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (2) lowered the catalytic transition free energy of the lit state relative to the dark state.
LOV2 photoactivation lowered the catalytic transition free energy of the lit state relative to the dark state in the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (2) lowered the catalytic transition free energy of the lit state relative to the dark state.
LOV2 photoactivation lowered the catalytic transition free energy of the lit state relative to the dark state in the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (2) lowered the catalytic transition free energy of the lit state relative to the dark state.
LOV2 photoactivation lowered the catalytic transition free energy of the lit state relative to the dark state in the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (2) lowered the catalytic transition free energy of the lit state relative to the dark state.
LOV2 photoactivation lowered the catalytic transition free energy of the lit state relative to the dark state in the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (2) lowered the catalytic transition free energy of the lit state relative to the dark state.
LOV2 photoactivation lowered the catalytic transition free energy of the lit state relative to the dark state in the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (2) lowered the catalytic transition free energy of the lit state relative to the dark state.
LOV2 photoactivation lowered the catalytic transition free energy of the lit state relative to the dark state in the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (2) lowered the catalytic transition free energy of the lit state relative to the dark state.
LOV2 photoactivation lowered the catalytic transition free energy of the lit state relative to the dark state in the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (2) lowered the catalytic transition free energy of the lit state relative to the dark state.
LOV2 photoactivation lowered the catalytic transition free energy of the lit state relative to the dark state in the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (2) lowered the catalytic transition free energy of the lit state relative to the dark state.
LOV2 photoactivation lowered the catalytic transition free energy of the lit state relative to the dark state in the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (2) lowered the catalytic transition free energy of the lit state relative to the dark state.
LOV2 photoactivation lowered the catalytic transition free energy of the lit state relative to the dark state in the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (2) lowered the catalytic transition free energy of the lit state relative to the dark state.
LOV2 photoactivation lowered the catalytic transition free energy of the lit state relative to the dark state in the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (2) lowered the catalytic transition free energy of the lit state relative to the dark state.
LOV2 photoactivation lowered the catalytic transition free energy of the lit state relative to the dark state in the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (2) lowered the catalytic transition free energy of the lit state relative to the dark state.
LOV2 photoactivation lowered the catalytic transition free energy of the lit state relative to the dark state in the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (2) lowered the catalytic transition free energy of the lit state relative to the dark state.
LOV2 photoactivation thermally destabilized the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (1) thermally destabilized the fusion
LOV2 photoactivation thermally destabilized the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (1) thermally destabilized the fusion
LOV2 photoactivation thermally destabilized the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (1) thermally destabilized the fusion
LOV2 photoactivation thermally destabilized the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (1) thermally destabilized the fusion
LOV2 photoactivation thermally destabilized the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (1) thermally destabilized the fusion
LOV2 photoactivation thermally destabilized the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (1) thermally destabilized the fusion
LOV2 photoactivation thermally destabilized the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (1) thermally destabilized the fusion
LOV2 photoactivation thermally destabilized the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (1) thermally destabilized the fusion
LOV2 photoactivation thermally destabilized the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (1) thermally destabilized the fusion
LOV2 photoactivation thermally destabilized the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (1) thermally destabilized the fusion
LOV2 photoactivation thermally destabilized the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (1) thermally destabilized the fusion
LOV2 photoactivation thermally destabilized the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (1) thermally destabilized the fusion
LOV2 photoactivation thermally destabilized the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (1) thermally destabilized the fusion
LOV2 photoactivation thermally destabilized the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (1) thermally destabilized the fusion
LOV2 photoactivation thermally destabilized the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (1) thermally destabilized the fusion
LOV2 photoactivation thermally destabilized the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (1) thermally destabilized the fusion
LOV2 photoactivation thermally destabilized the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (1) thermally destabilized the fusion
The DHFR/LOV2 fusion was marginally stable at physiological temperatures.
We found that the DHFR/LOV2 fusion was marginally stable at physiological temperatures.
The DHFR/LOV2 fusion was marginally stable at physiological temperatures.
We found that the DHFR/LOV2 fusion was marginally stable at physiological temperatures.
The DHFR/LOV2 fusion was marginally stable at physiological temperatures.
We found that the DHFR/LOV2 fusion was marginally stable at physiological temperatures.
The DHFR/LOV2 fusion was marginally stable at physiological temperatures.
We found that the DHFR/LOV2 fusion was marginally stable at physiological temperatures.
The DHFR/LOV2 fusion was marginally stable at physiological temperatures.
We found that the DHFR/LOV2 fusion was marginally stable at physiological temperatures.
The DHFR/LOV2 fusion was marginally stable at physiological temperatures.
We found that the DHFR/LOV2 fusion was marginally stable at physiological temperatures.
The DHFR/LOV2 fusion was marginally stable at physiological temperatures.
We found that the DHFR/LOV2 fusion was marginally stable at physiological temperatures.
The DHFR/LOV2 fusion was marginally stable at physiological temperatures.
We found that the DHFR/LOV2 fusion was marginally stable at physiological temperatures.
The DHFR/LOV2 fusion was marginally stable at physiological temperatures.
We found that the DHFR/LOV2 fusion was marginally stable at physiological temperatures.
The DHFR/LOV2 fusion was marginally stable at physiological temperatures.
We found that the DHFR/LOV2 fusion was marginally stable at physiological temperatures.
The DHFR/LOV2 fusion was marginally stable at physiological temperatures.
We found that the DHFR/LOV2 fusion was marginally stable at physiological temperatures.
The DHFR/LOV2 fusion was marginally stable at physiological temperatures.
We found that the DHFR/LOV2 fusion was marginally stable at physiological temperatures.
The DHFR/LOV2 fusion was marginally stable at physiological temperatures.
We found that the DHFR/LOV2 fusion was marginally stable at physiological temperatures.
The DHFR/LOV2 fusion was marginally stable at physiological temperatures.
We found that the DHFR/LOV2 fusion was marginally stable at physiological temperatures.
The DHFR/LOV2 fusion was marginally stable at physiological temperatures.
We found that the DHFR/LOV2 fusion was marginally stable at physiological temperatures.
The DHFR/LOV2 fusion was marginally stable at physiological temperatures.
We found that the DHFR/LOV2 fusion was marginally stable at physiological temperatures.
The DHFR/LOV2 fusion was marginally stable at physiological temperatures.
We found that the DHFR/LOV2 fusion was marginally stable at physiological temperatures.
Approval Evidence
1 source7 linked approval claimsfirst-pass slug dhfr-lov2-fusion
we used LOV2 to create a light-regulated Dihydrofolate Reductase (DHFR) enzyme
Source:
correlationsupports
Many allostery-tuning mutations showed a negative correlation between light-induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Many of the allostery tuning mutations showed a negative correlation between the light induced change in thermal stability and catalytic activity, suggesting an activity-stability tradeoff.
Source:
mechanistic associationsupports
Local disorder is associated with enhanced catalysis in the engineered photoswitch.
Local disorder is associated with enhanced catalysis in an engineered photoswitch
Source:
mechanistic modelsupports
The energetic effect of light activation on transition state free energy was composed of a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
The energetic effect of light activation on the transition state free energy was composed of two opposing forces: a favorable reduction in the enthalpic transition state barrier offset by an entropic penalty.
Source:
mutation effectsupports
Allostery-tuning mutations in DHFR modulated the enthalpy-entropy tradeoff by either accentuating the enthalpic benefit or minimizing the entropic penalty, but not both.
Allostery-tuning mutations in DHFR acted through this tradeoff, either accentuating the enthalpic benefit or minimizing the entropic penalty but never improving both.
Source:
photoactivation effectsupports
LOV2 photoactivation lowered the catalytic transition free energy of the lit state relative to the dark state in the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (2) lowered the catalytic transition free energy of the lit state relative to the dark state.
Source:
photoactivation effectsupports
LOV2 photoactivation thermally destabilized the DHFR/LOV2 fusion.
LOV2 photoactivation simultaneously: (1) thermally destabilized the fusion
Source:
stability propertysupports
The DHFR/LOV2 fusion was marginally stable at physiological temperatures.
We found that the DHFR/LOV2 fusion was marginally stable at physiological temperatures.
Source:
Comparisons
Source-backed strengths
The source literature supports that the fusion produces a light-regulated DHFR and provides mechanistic analysis linking enhanced catalysis to local disorder. It also reports a mechanistic model in which light lowers the enthalpic transition-state barrier while incurring an entropic penalty, and notes a negative correlation between light-induced thermal stability changes and catalytic activity across many allostery-tuning mutations.
Compared with engineered focal adhesion kinase two-input gate
DHFR/LOV2 fusion and engineered focal adhesion kinase two-input gate address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; shared mechanisms: allosteric switching; same primary input modality: light
Compared with OptoORAI1
DHFR/LOV2 fusion and OptoORAI1 address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; shared mechanisms: allosteric switching; same primary input modality: light
Compared with photoactivatable diaphanous autoregulatory domain
DHFR/LOV2 fusion and photoactivatable diaphanous autoregulatory domain address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; shared mechanisms: allosteric switching; same primary input modality: light
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