Source 1review2022Frontiers in Bioengineering and Biotechnology
Toolkit/synthetic biology approaches for opto-protein development
synthetic biology approaches for opto-protein development
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Synthetic biology approaches for opto-protein development are protein engineering strategies for creating and optimizing non-neuronal light-regulatable proteins. The cited review describes modifying photosensitive domains and fusing them to effector domains to generate light-controllable protein functions.
Usefulness & Problems
Why this is useful
These approaches are useful for developing novel optogenetic proteins whose activity can be regulated by light. The review specifically positions protein engineering and synthetic biology as means to aid the development and optimization of non-neuronal opto-proteins.
Problem solved
These approaches address the engineering problem of converting proteins into light-controllable systems. The supplied evidence specifically identifies modification of photosensitive domains and fusion to effector domains as strategies for generating light-regulated function.
Problem links
Need precise spatiotemporal control with light input
DerivedSynthetic biology approaches for opto-protein development comprise protein engineering strategies for creating and optimizing non-neuronal light-regulatable proteins. The cited review describes modification of photosensitive domains and fusion of these domains to effector proteins to generate light-controllable functions.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: builderswitch architecture: uncaging
Implementation is described at the level of construct design, namely modification of photosensitive domains and their fusion to effector domains. The provided evidence does not specify cofactors, expression systems, delivery methods, or domain architectures beyond this general strategy.
The supplied evidence is review-level and does not identify a single defined construct, target protein class, or validated performance metric. No specific wavelengths, host organisms, dynamic ranges, kinetics, or comparative experimental results are provided in the evidence here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
Approval Evidence
1 source1 linked approval claimfirst-pass slug synthetic-biology-approaches-for-opto-protein-development
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins).
Source:
review scopesupports
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
Source:
Comparisons
Source-backed strengths
A key strength is conceptual generality: the review discusses both photosensitive-domain modification and fusion to effector domains as design strategies for light control. The evidence supports relevance to both development and optimization of novel non-neuronal opto-proteins, but does not provide quantitative performance benchmarks.
Compared with CRY2 C-terminal tail
synthetic biology approaches for opto-protein development and CRY2 C-terminal tail address a similar problem space.
Shared frame: shared mechanisms: allosteric switching; same primary input modality: light
Compared with doxycycline-dependent photoactivated gene expression
synthetic biology approaches for opto-protein development and doxycycline-dependent photoactivated gene expression address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with oligomerization reactions
synthetic biology approaches for opto-protein development and oligomerization reactions address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Ranked Citations
- 1.