In this study, Arabidopsis thaliana AtC3H26, a non-TZF CCCH protein and paralog of AtC3H3, was characterized, and its role as a ribonuclease (RNase) in salt tolerance and phosphate (Pi) homeostasis was demonstrated.
First-pass extracted concept
AtC3H26
Aliases
Arabidopsis thaliana AtC3H26
Evidence Snippets
Supporting Sources
Linked Claims
AtC3H26 degrades RNA substrates in vitro in a dose-dependent manner.
In vitro assays confirmed that AtC3H26 degraded RNA substrates in a dose-dependent manner
AtC3H26 expression is rapidly induced by salt, mannitol, and abscisic acid.
AtC3H26 expression was rapidly induced by salt, mannitol, and abscisic acid (ABA)
AtC3H26 functions as a ribonuclease in Arabidopsis.
its role as a ribonuclease (RNase) in salt tolerance and phosphate (Pi) homeostasis was demonstrated
AtC3H26 modulates both ABA-dependent and ABA-independent stress marker genes.
AtC3H26 modulated both ABA-dependent and -independent stress marker genes
AtC3H26 overexpression enhances salt tolerance but does not confer drought resistance.
its overexpression (OX) lines exhibited enhanced salt tolerance but not drought resistance
AtC3H26 overexpression plants accumulate higher phosphate levels than wild type plants.
AtC3H26 OX plants accumulated higher Pi levels than those in wild-type plants
small RNA-sequencing of AtC3H26 overexpression lines identified downregulation of miR399 and miR827.
small RNA-sequencing identified the downregulation of miR399 and miR827
AtC3H26 is established as a novel cytoplasmic RNase in Arabidopsis that coordinates RNA turnover with stress and nutrient signaling.
Collectively, these findings establish AtC3H26 as a novel cytoplasmic RNase in Arabidopsis that coordinates RNA turnover with stress and nutrient signaling.
mRNA-sequencing of AtC3H26 overexpression lines showed upregulation of phosphate starvation-responsive genes including SPX1, PS2/PECP2, and SRG3/GDPD1.
mRNA-sequencing of OX lines revealed substantial upregulation of Pi starvation-responsive genes, including SPX1, PS2/PECP2, and SRG3/GDPD1