Single-cell characterization of bacterial optogenetic Cre recombinases
First-pass extracted concept
bacterial optogenetic Cre recombinases
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Linked Claims
General trends matched expectations for light-dependent recombination, but efficiency and timing varied substantially from cell to cell.
Quoted textsource-backed
Although general trends match expectations for light-dependent recombination, we found substantial variation in the efficiency and timing of recombinase activity from cell to cell.
The findings suggest criteria for selecting optogenetic recombinase systems and indicate areas for optimization to improve single-cell capabilities of bacterial optogenetic tools.
Quoted textsource-backed
These findings suggest critical criteria for selecting optogenetic recombinase systems and indicate areas for optimization to improve single-cell capabilities of bacterial optogenetic tools.
The study quantifies recombination efficiency, expression variability, and activation dynamics using reporters that change fluorescence or antibiotic resistance after light-induced Cre activity.
Quoted textsource-backed
We quantify recombination efficiency, expression variability, and activation dynamics using reporters which produce changes in fluorescence or antibiotic resistance following light-induced Cre activity.
Optogenetic recombinase performance can be reporter-dependent.
Quoted textsource-backed
Our results indicate that optogenetic recombinase performance can be reporter-dependent.
Single-cell analysis revealed highly heterogeneous optogenetic recombinase activity across cells.
Quoted textsource-backed
Further, single-cell analysis revealed highly heterogeneous activity across cells.