improved dystrophin expression in muscle fibres with a ubiquitous CAG promoter (1.61-fold in CAG vs. MHCK7, p < 0.0001)
First-pass extracted concept
CAG promoter
Candidate: concept label2 source documents4 linked claims
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Linked Claims
In mdx mice, the CAG promoter produced higher dystrophin expression in muscle fibres than MHCK7 during initial promoter screening.
Quoted textsource-backed
improved dystrophin expression in muscle fibres with a ubiquitous CAG promoter (1.61-fold in CAG vs. MHCK7, p < 0.0001) in mdx mice
Either of the two GC-rich regions was sufficient to render the plasmid incompatible with P3a mutagenesis.
Quoted textsource-backed
deletion mutagenesis revealed that either of the two GC-rich regions is sufficient for rendering the plasmid incompatible with P3a mutagenesis
GC-rich regions in the synthetic CAG promoter and KAT2B coding region may form G-quadruplexes and hinder plasmid denaturation during PCR.
Quoted textsource-backed
two GC-rich regions located within the synthetic CAG promoter and the KAT2B coding region may form guanine (G)-quadruplexes and hinder plasmid denaturation during PCR
Only P3b mutagenesis worked efficiently with several widely used sgRNA/Cas9 expression vectors containing the CAG promoter and with a CDK13 expression vector containing a GC-rich coding fragment.
Quoted textsource-backed
only P3b mutagenesis worked efficiently with several widely used sgRNA/Cas9 expression vectors, which contain the CAG promoter, and with an expression vector for CDK13, which possesses an intrinsically disordered domain encoded by a GC-rich DNA fragment