This study framework measures protein and metabolite abundance variation in neurologically normal human CSF across several analytical platforms. It is presented as a baseline reference effort rather than a single engineered tool.
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cerebrospinal fluid proteomics and metabolomics baseline variation profiling
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CSF proteins and metabolites with high inter-individual variation should be treated cautiously as candidate biomarkers because part of case-control differences may reflect natural biological variation rather than disease.
The metabolome of neurologically normal human CSF showed more limited variation than the proteome in this study.
Understanding biological variation of proteins and metabolites in CSF from neurologically normal individuals is essential for reliable interpretation of CNS biomarker discovery studies.
GC-MS, NMR, and LC-MS/MS metabolomics of neurologically normal human CSF detected more than 100 metabolites, with inter-individual relative standard deviations ranging from 12% to 70%.
In neurologically normal human CSF, 126 common proteins measured by ESI-Orbitrap showed broad inter-individual variation with relative standard deviations ranging from 18% to 148%.
Technical variation for the 126 CSF proteins measured by ESI-Orbitrap was between 15% and 30%.
Technical variation was less than 20% for almost all CSF metabolites measured in this study.