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chemically inducible CRISPR/Cas9 circuits

Candidate: concept label1 source documents4 linked claims
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Evidence Snippets

Chemically-inducible CRISPR/Cas9 circuits for ultra-high dynamic range gene perturbation.
Evidence 1Source 1DOIPubMedprovenance

Supporting Sources

Linked Claims

Claim 1application scopesupports2025Source 1DOIPubMed

The upgraded suite of inducible CRISPR systems has broad applications across numerous cell types and experimental conditions.

Quoted textsource-backed
This upgraded suite of inducible CRISPR systems has broad applications for numerous cell types and experimental conditions.
Claim 2problem statementsupports2025Source 1DOIPubMed

Widely used Tet-On systems often show leaky Cas9 expression, causing unintended edits and weak activity upon induction.

Quoted textsource-backed
However, widely-used Tet-On systems often show leaky Cas9 expression, leading to unintended edits, as well as weak activity upon induction.
Claim 3safety rationalesupports2025Source 1DOIPubMed

Leaky Cas9 nuclease activity can cause cumulative DNA damage and degradation of the target cell genome over time.

Quoted textsource-backed
Leakiness can be problematic in the context of Cas9 nuclease activity, which may result in cumulative DNA damage and degradation of the target cell genome over time.
Claim 4tool capabilitysupports2025Source 1DOIPubMed

The authors established transgenic platforms that minimize Cas9 functionality in the OFF-state while maximizing uncompromised ON-state gene editing efficiency.

Quoted textsource-backed
To overcome these deficiencies, we have established transgenic platforms that minimize Cas9 functionality in the OFF-state along with maximized and uncompromised ON-state gene editing efficiency.