The abstract presents chemogenetics as a precision-enhancing technique for epilepsy therapies that enables greater control over neuronal activity.
First-pass extracted concept
chemogenetics
Candidate: concept label17 source documents49 linked claims
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Aliases
chemogenetic approaches
Extracted Explainers
What the tool is doing
The abstract names chemogenetics as a highlighted technology in neural regeneration.
Chemogenetics is presented as a method to manipulate neuronal activity and thereby test the functional contribution of transplanted cells.
Chemogenetics is described as a controllable, low-invasive approach for modulating neuronal activity and manipulating selective brain circuits.
Chemogenetics is described as a tool used to acutely manipulate astrocytes in vivo.
Chemogenetics is described as a neuromodulation technique whose chronic character is considered a key asset. The review focuses on its long-term application and associated hurdles.
Chemogenetics enables control of neural activity in targeted neurons using engineered receptors or ion channels plus synthetic ligands. In this review, it is framed as a strategy for investigating brain function and behavior.
Chemogenetics is described as an approach for controlling nerve cell electrical activity using delivered exogenous, genetically encoded molecules responsive to external stimuli.
Chemogenetics is described as part of a set of genetically encoded approaches used to modulate and monitor activity with high cellular specificity. The review presents it as promising for neurogastroenterology.
Chemogenetics is presented as an experimental approach for controlling defined cell populations using engineered receptors. In this review, DREADDs are highlighted as a specific chemogenetic application.
Chemogenetics is the broader technology area in which the review situates DREADDs. It is described as transforming how GPCR involvement is deconstructed in physiological and translational settings.
Resources required
The abstract only supports that it is used as an astrocyte manipulation tool in vivo; specific receptors, ligands, or delivery details are not provided.
The abstract states that chemogenetic control uses engineered G protein-coupled receptors or ligand-gated ion channels together with exogenously inert synthetic ligands.
The abstract states that the approach depends on delivery of exogenous, genetically encoded stimulus-sensitive molecules into nervous tissue.
The abstract supports the need for genetically encoded tools and cell-type-specific transgene targeting strategies.
The abstract indicates that chemogenetics relies on engineered GPCRs and compounds that control them.
What problem it solves
It is described as helping make therapeutic interventions more precise.
It is framed as part of the recent biotechnology breakthroughs promoting development in the field.
It supports functional interrogation of grafted-cell integration in host neural networks.
It addresses the need for selective circuit manipulation in neuroscience research and is framed as potentially useful for future clinical applications.
It helps establish causal links between astrocyte activity and behavioral outcomes.
It provides a research platform for repeated, long-term neuromodulation and for probing deregulated neural circuits.
It addresses the challenge of identifying neuronal mechanisms responsible for targeted behaviors induced by drug application.
It addresses specific external control of excitation or inhibition in electrically excitable cells.
It provides selective access to defined cell populations for physiology studies in the gut and related neural circuits.
It provides selective access to neural circuits that can then be linked to behavioral outputs.
What it does not solve
The abstract indicates that widespread application in nonhuman primates has not yet occurred and that important limitations remain.
The abstract does not indicate that chemogenetics by itself resolves astrocyte heterogeneity or specifies the exact downstream mechanisms.
The abstract states that acute results cannot simply be extrapolated to chronic experiments and that important chronic-use hurdles remain unresolved.
The abstract does not claim that chemogenetics alone fully resolves neuropathological mechanisms or therapeutic translation.
The abstract does not detail specific gut-specific limitations or operational tradeoffs for chemogenetics.
Alternatives
No explicit alternative neuromodulation methods are named in the abstract.
Optogenetics is named alongside chemogenetics as another acute astrocyte manipulation approach.
Optogenetics is explicitly mentioned as a comparison point.
The abstract contrasts engineered G protein-coupled receptors with ligand-gated ion channel approaches within the broader chemogenetics toolbox.
The review frames chemogenetics alongside optogenetics and thermogenetics as complementary rather than competing approaches.
Optogenetics is discussed in parallel as another major genetically encoded control and monitoring modality.
The abstract does not name a direct alternative platform, but treats DREADDs as one member of the broader chemogenetics space.
Evidence Snippets
cutting-edge techniques such as optogenetics, chemogenetics, and clustered regularly interspaced short palindromic repeat-based gene editing are enhancing the precision of these therapies, enabling greater control over neuronal activity
Therefore, this review aims to investigate the current understanding of the neural circuit mechanisms in epilepsy based on various technologies, including electroencephalography, magnetic resonance imaging, optogenetics, chemogenetics, deep brain stimulation, and brain-computer interfaces.
This review categorizes neuromodulation techniques into genetic neuromodulation methods (including optogenetics, chemogenetics, sonogenetics, and magnetogenetics)
This review highlights the latest cutting-edge technologies driving progress in the field, including ... chemogenetics...
Emerging tools such as photosensitive pharmaceuticals, chemogenetics, and optogenetics enable the spatiotemporal manipulation of structures, dynamics, post-translational modifications, and cross-linking with actin filaments in target microtubule subtypes.
From precise manipulation of neuronal activity to pinpoint the functional contribution of transplanted cells by using opto- and chemo-genetics
Due to its low invasiveness and controllability, chemogenetic approaches offer a highly attractive option to modulate neuronal activity in basic research and future clinical applications.
Chemogenetics enables precise, non-invasive, and reversible modulation of neural activity via the activation of engineered receptors that are pharmacologically selective to endogenous or exogenous ligands.
Using tools to acutely manipulate astrocytes, such as optogenetics or chemogenetics, studies reviewed here have demonstrated a causal role for astrocytes in sleep, memory, sensorimotor behaviors, feeding, fear, anxiety, and cognitive processes like attention and behavioral flexibility.
Optogenetics and chemogenetics drive brain research forward by exploring causal relationships among different brain regions.
The chronic character of chemogenetics has been put forward as one of the assets of the technique, particularly in comparison to optogenetics.
The development of chemogenetic approaches has allowed researchers to control neural activities in targeted neurons through a toolbox, including engineered G protein-coupled receptors or ligand-gated ion channels together with exogenously inert synthetic ligands.
These approaches include optogenetics (overviewed in Part I), as well as chemogenetics and thermogenetics (described here, in Part II)
Touchscreen tasks are also readily used with cutting-edge neuroscientific methods that are difficult to do in humans such as optogenetics, chemogenetics, neurophysiology and calcium imaging (using miniscopes).
Optogenetics and chemogenetics comprise a wide variety of applications in which genetically encoded actuators and indicators are used to modulate and monitor activity with high cellular specificity.
In recent years, the selective targeting of specific neural circuits has been made possible with the development of new experimental approaches, including chemogenetics. This technique allows for the control of molecularly defined subsets of cells through engineered G protein-coupled receptors (GPCRs), which have the ability to activate or silence neuronal firing.
In the past decade, emerging synthetic biology technologies such as chemogenetics have dramatically transformed how pharmacologists and systems biologists deconstruct the involvement of G protein-coupled receptors (GPCRs) in a myriad of physiological and translational settings.
Supporting Sources
Linked Claims
Optogenetics, chemogenetics, and CRISPR-based gene editing are enhancing the precision of epilepsy therapies and enabling greater control over neuronal activity.
Quoted textsource-backed
cutting-edge techniques such as optogenetics, chemogenetics, and clustered regularly interspaced short palindromic repeat-based gene editing are enhancing the precision of these therapies, enabling greater control over neuronal activity
The review investigates neural circuit mechanisms in epilepsy using evidence from electroencephalography, magnetic resonance imaging, optogenetics, chemogenetics, deep brain stimulation, and brain-computer interfaces.
Quoted textsource-backed
Therefore, this review aims to investigate the current understanding of the neural circuit mechanisms in epilepsy based on various technologies, including electroencephalography, magnetic resonance imaging, optogenetics, chemogenetics, deep brain stimulation, and brain-computer interfaces.
Neuromodulation techniques have shown significant advancements in treating neurological and psychiatric disorders.
The review categorizes neuromodulation techniques into genetic methods and non-genetic methods.
Fully harnessing the therapeutic potential of neuromodulation requires integration and innovation in technologies, optimization of delivery methods, improvement of mediums, and evaluation of toxicity.
The review highlights optogenetics, chemogenetics, 3D culture models, gene editing, single-cell sequencing, and 3D imaging as cutting-edge technologies driving progress in neural regeneration.
Quoted textsource-backed
This review highlights the latest cutting-edge technologies driving progress in the field, including optogenetics, chemogenetics, three-dimensional (3D) culture models, gene editing, single-cell sequencing, and 3D imaging.
The convergence of multidisciplinary approaches in neural regeneration is presented as having potential to enable more precise, efficient, and personalized therapeutic strategies and improve functional recovery.
Quoted textsource-backed
The convergence of these multidisciplinary approaches holds immense potential for developing transformative treatments for neural injuries and neurological disorders, ultimately improving functional recovery.
Photosensitive pharmaceuticals, chemogenetics, and optogenetics enable spatiotemporal manipulation of structures, dynamics, post-translational modifications, and actin-filament cross-linking in target microtubule subtypes.
How different microtubule subtypes regulate cellular architectures and activities remains largely unexplored.
The molecular toolbox reviewed holds promise for elucidating the impact of cell therapy on neural circuitry and guiding development of more effective treatments for neurological disorders.
This review summarizes the design rationale and applications of emerging approaches for manipulating microtubule dynamics and post-translational modifications in cellular contexts.
Optogenetics and chemogenetics can be used to precisely manipulate neuronal activity to pinpoint the functional contribution of transplanted cells.
Most chemogenetics studies have been conducted in rodent models, and wide application in nonhuman primates has not yet occurred.
Chemogenetics enables precise, non-invasive, and reversible modulation of neural activity through activation of engineered receptors that are pharmacologically selective to endogenous or exogenous ligands.
In vivo studies reviewed in this paper indicate that acute astrocyte manipulation with optogenetics or chemogenetics demonstrates a causal role for astrocytes in multiple behaviors and cognitive processes.
Quoted textsource-backed
Here we review recent findings on the active role of astrocytes in behavioral modulation with a focus on in vivo studies, primarily in mice. Using tools to acutely manipulate astrocytes, such as optogenetics or chemogenetics, studies reviewed here have demonstrated a causal role for astrocytes in sleep, memory, sensorimotor behaviors, feeding, fear, anxiety, and cognitive processes like attention and behavioral flexibility.
Chemogenetics is presented as having chronic character as an asset compared with optogenetics.
Quoted textsource-backed
The chronic character of chemogenetics has been put forward as one of the assets of the technique, particularly in comparison to optogenetics.
Chemogenetics have revolutionized neuroscience research by facilitating manipulations of selective brain circuits.
Optogenetics and chemogenetics are described as advancing brain research by enabling exploration of causal relationships among brain regions.
Quoted textsource-backed
Optogenetics and chemogenetics drive brain research forward by exploring causal relationships among different brain regions.
Most chemogenetic studies have focused on acute applications, while repeated long-term neuromodulation studies have increased only in recent years.
Quoted textsource-backed
Yet, the vast majority of chemogenetic studies have focused on acute applications, while repeated, long-term neuromodulation has only been booming in the past few years.
Effects of acute neuromodulation cannot be extrapolated to chronic chemogenetic experiments.
Quoted textsource-backed
It becomes increasingly clear that chronic neuromodulation warrants caution and that the effects of acute neuromodulation cannot be extrapolated towards chronic experiments.
Chronic chemogenetic application has uncovered various hurdles.
Quoted textsource-backed
Unfortunately, together with the rising number of studies, various hurdles have also been uncovered, especially in relation to its chronic application.
Current limitations of chemogenetics are barriers to more widespread use in nonhuman primates.
The review discusses chemogenetics as a precision medicine-based neuromodulation strategy with both potential and challenges.
Chemogenetic investigations are concentrated on behavioral readouts, while molecular signatures after chronic neuromodulation remain underexplored and may provide new insights.
Quoted textsource-backed
For example, most investigations are concentrated on behavioral read-outs, whereas dissecting the underlying molecular signature after (chronic) neuromodulation could reveal novel insights in terms of basic neuroscience and deregulated neural circuits.
The scoping review identified applications of chemogenetics that led to reversal of molecular and behavioral deficits in studies relevant to neuropsychiatric diseases and disorders.
With recent advances in therapeutic gene delivery, chemogenetics is poised to support novel interventions against neuropsychiatric diseases and disorders.
Chemogenetic approaches offer a low-invasive and controllable option to modulate neuronal activity in basic research and future clinical applications.
Minimally invasive methods for brain tissue stimulation are becoming a basic element in the neuroscience toolbox for direct investigation of complex neuronal systems.
Quoted textsource-backed
minimally invasive methods for brain tissue stimulation are becoming the basic element in the toolbox of those involved in the field
Chemogenetic approaches allow researchers to control neural activities in targeted neurons using engineered G protein-coupled receptors or ligand-gated ion channels together with exogenously inert synthetic ligands.
Optogenetics, chemogenetics, and thermogenetics are presented as complementary rather than competing techniques.
Quoted textsource-backed
The latter circumstance is an indication that these are rather complementary than competing techniques.
Chemogenetics has had a significant impact on basic neuroscience by improving understanding of relationships between brain activity and behavior with cell- and circuit-specific resolution.
Targeted manipulation of electrically excitable cells aims to control electrical activity to either excite cells and generate action potentials or inhibit cells and suppress excitatory currents.
Quoted textsource-backed
In the case of electrically excitable cells, the aim of the manipulation is to control the cells' electrical activity, with the result being either excitation with subsequent generation of an action potential or inhibition and suppression of the excitatory currents.
Chemogenetics is potentially useful for deconstructing neuropathological mechanisms of mental diseases and their regulation by drugs, and may contribute to transformative therapeutics.
This review describes genetically encoded approaches for targeted control of nerve cell electrical activity, focusing in Part II on chemogenetics and thermogenetics.
Quoted textsource-backed
In this review, we describe three approaches that are based on the delivery of exogenous, genetically encoded molecules sensitive to external stimuli into the nervous tissue. These approaches include optogenetics (overviewed in Part I), as well as chemogenetics and thermogenetics (described here, in Part II)
Touchscreen tasks are readily combined with optogenetics, chemogenetics, neurophysiology, and calcium imaging using miniscopes.
Although primarily developed in central nervous system research, optogenetic and chemogenetic methods hold strong promise for neurogastroenterology.
Quoted textsource-backed
Although innovation of optogenetic and chemogenetic methods has been primarily driven by researchers studying the central nervous system, these techniques also hold great promise to boost research in neurogastroenterology.
Optogenetic and chemogenetic techniques hold promise for advancing research in neurogastroenterology.
Quoted textsource-backed
these techniques also hold great promise to boost research in neurogastroenterology
Optogenetic and chemogenetic applications use genetically encoded actuators and indicators to modulate and monitor activity with high cellular specificity.
Quoted textsource-backed
Optogenetics and chemogenetics comprise a wide variety of applications in which genetically encoded actuators and indicators are used to modulate and monitor activity with high cellular specificity.
Progress in strategies for targeting transgene expression to specific cell types is an important enabler for optogenetic and chemogenetic applications.
Quoted textsource-backed
strategies to target transgene expression to specific cell types have also made much progress in the past 20 years
Successful implementation of optogenetic and chemogenetic techniques depends on advances in live imaging microscopy and optical technology.
Quoted textsource-backed
the successful implementation of optogenetic and chemogenetic techniques thrives thanks to ongoing advances in live imaging microscopy and optical technology
Successful implementation of optogenetic and chemogenetic techniques depends on advances in live imaging microscopy and optical technology.
Quoted textsource-backed
the successful implementation of optogenetic and chemogenetic techniques thrives thanks to ongoing advances in live imaging microscopy and optical technology
Development of genetically encoded optogenetic and chemogenetic tools has contributed substantially to understanding integrated physiology over the past 10 years.
Quoted textsource-backed
Over the past 10 years, development of these genetically encoded tools has contributed tremendously to our understanding of integrated physiology.
Development of genetically encoded optogenetic and chemogenetic tools has contributed substantially to understanding integrated physiology over the past decade.
Quoted textsource-backed
Over the past 10 years, development of these genetically encoded tools has contributed tremendously to our understanding of integrated physiology.
Optogenetics and chemogenetics use genetically encoded actuators and indicators to modulate and monitor activity with high cellular specificity.
Quoted textsource-backed
Optogenetics and chemogenetics comprise a wide variety of applications in which genetically encoded actuators and indicators are used to modulate and monitor activity with high cellular specificity.
The review covers available tool classes, strategies to target them to specific cell types in the gut wall, and the possibilities and limitations of optogenetic and chemogenetic technology in the gut with emphasis on the enteric nervous system.
Quoted textsource-backed
In this Review, we describe the different classes of tools that are currently available and give an overview of the strategies to target them to specific cell types in the gut wall. We discuss the possibilities and limitations of optogenetic and chemogenetic technology in the gut and provide an overview of their current use, with a focus on the enteric nervous system.
The review discusses possibilities, limitations, and current use of optogenetic and chemogenetic technology in the gut, with a focus on the enteric nervous system.
Quoted textsource-backed
We discuss the possibilities and limitations of optogenetic and chemogenetic technology in the gut and provide an overview of their current use, with a focus on the enteric nervous system.
Chemogenetic control through engineered GPCRs can activate or silence neuronal firing in molecularly defined subsets of cells.
Coupling chemogenetics with imaging techniques to monitor neural activity in freely moving animals can support deconstruction of whole-brain networks underlying behavioral states.
Chemogenetic technologies are presented as having potential utility for transformative therapeutics.