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concerted chromophore isomerization and protonation mechanism

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Aliases

concerted mechanism

Evidence Snippets

chromophore cis to trans photoswitching involves a molecular mechanism where stereochemical isomerization and chromophore protonation occur in a coordinated way. Such a "concerted" mechanism is, in our opinion, at the basis of efficient photochromic behavior and might be activated by the E222Q mutation.
Evidence 1Source 1DOIPubMedprovenance

Supporting Sources

Linked Claims

Claim 1mechanistic hypothesissupports2010Source 1DOIPubMed

A concerted isomerization-protonation mechanism may underlie efficient photochromic behavior and may be activated by the E222Q mutation.

Quoted textsource-backed
Such a "concerted" mechanism is, in our opinion, at the basis of efficient photochromic behavior and might be activated by the E222Q mutation.
Claim 2mechanistic hypothesissupports2010Source 1DOIPubMed

Substitution of glutamic acid 222 with glutamine presumably rewires proton pathways around the GFP chromophore and thereby affects photochromic properties.

Quoted textsource-backed
Glutamic acid 222 is known to play a pivotal role in the inner proton wires that involve the GFP chromophore and the surrounding residues. Hence its substitution with an isosteric but non-ionizable residue presumably leads to a extensive rewiring of proton pathways around the chromophore, which has a deep effect also on the photochromic properties.
Claim 3mechanistic summarysupports2010Source 1DOIPubMed

Chromophore cis to trans photoswitching in E222Q GFP mutants involves coordinated stereochemical isomerization and chromophore protonation.

Quoted textsource-backed
we show, by means of flash-photolysis experiments, that chromophore cis to trans photoswitching involves a molecular mechanism where stereochemical isomerization and chromophore protonation occur in a coordinated way.
Claim 4mechanistic summarysupports2010Source 1DOIPubMed

Photochromicity in fluorescent proteins usually stems from reversible cis-trans photoisomerization of the chromophore.

Quoted textsource-backed
From a mechanistic point of view, photochromicity usually stems from the reversible cis-trans photoisomerization of the chromophore.