First-pass extracted concept

domain insertion engineering

Candidate: concept label2 source documents4 linked claims

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Evidence Snippets

Domain insertion engineering is a powerful approach to juxtapose otherwise separate biological functions, resulting in proteins with new-to-nature activities.

Evidence 1Source 1DOI PubMedprovenance

Domain insertion engineering is a promising approach to recombine the functions of evolutionarily unrelated proteins.

Evidence 2Source 2DOI PubMedprovenance

Supporting Sources

Source 1primary paper2025MEDDOI PubMed

1 evidence snippet 1 linked claim Claim 1

Source 2primary paper2023Advanced ScienceDOI PubMed

1 evidence snippet 3 linked claims Claim 1 Claim 2 Claim 3

Linked Claims

Claim 1impactsupports2025Source 1DOI PubMed

The work enables one-shot domain insertion engineering and substantially accelerates design of customized allosteric proteins.

Claim 2capabilitysupports2023Source 2DOI PubMed

Domain insertion engineering can recombine functions of evolutionarily unrelated proteins.

Claim 3mechanistic design principlesupports2023Source 2DOI PubMed

Insertion of light-switchable receptor domains into a selected effector protein can yield allosteric effectors with light-dependent activity.

Claim 4study conclusionsupports2023Source 2DOI PubMed

The study revealed determinants of domain insertion tolerance and yielded multimodally switchable proteins with unique functional properties.