we used a genetically encoded Ff6rster resonance energy transfer (FRET)-based biosensor to record b3-secretase activity, aiming to determine if naturally occurring cell-by-cell variations in endogenous b3-secretase activity are associated with phagocytic activity.
First-pass extracted concept
endogenous b3-secretase activity
Candidate: concept label1 source documents4 linked claims
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Cells with reduced phagocytic activity exhibit decreased endogenous b3-secretase activity in complementary biochemical analysis using Zymosan bioparticles and FACS.
Quoted textsource-backed
Complementary biochemical analysis, utilizing Zymosan bioparticles and fluorescence-activated cell sorting (FACS), further demonstrated that cells with reduced phagocytic activity exhibited decreased endogenous b3-secretase activity.
Endogenous b3-secretase activity varies among individual BV-2 microglial cells.
Quoted textsource-backed
Using the Notch1 N100 Y-T biosensor, we found that the regulation of endogenous b3-secretase activity varies among individual BV-2 microglial cells.
Lower endogenous b3-secretase activity is associated with impaired phagocytosis of E. coli bioparticles in BV-2 microglial cells.
Quoted textsource-backed
Our multiplexed time-lapse imaging revealed that the phagocytosis of E. coli bioparticles was impaired in cells with lower b3-secretase activity compared to those with higher activity.
Microglial phagocytic activity is closely linked to b3-secretase.
Quoted textsource-backed
Collectively, our confirmatory study supports previous findings that microglial phagocytic activity is closely linked to b3-secretase and emphasizes the essential role of b3-secretase in microglia.