First-pass extracted concept

Förster resonance energy transfer

Candidate: concept label4 source documents5 linked claims
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Aliases

fluorescence resonance energy transfer, FRET

Extracted Explainers

What the tool is doing

FRET is presented as a fluorescence-based technology for visualizing signaling molecules in live cells. The review frames it as a high-spatiotemporal-resolution measurement approach.

Source 2DOIPubMed

Resources required

The abstract indicates that FRET studies require methods to measure FRET changes in biological contexts and commonly use fluorescent proteins as donor and acceptor fluorophores.

Source 2DOIPubMed

What problem it solves

It helps monitor signaling events in living cells with spatial and temporal detail.

Source 2DOIPubMed

What it does not solve

The abstract does not specify particular signaling pathways, analytes, or non-fluorescent measurement problems that FRET cannot address.

Source 2DOIPubMed

Alternatives

No direct alternative live-cell signaling readout technologies are named in the abstract.

Source 2DOIPubMed

Evidence Snippets

applications based on fluorescence resonance energy transfer (FRET) with fluorescent materials
Evidence 1Source 1DOIPubMedprovenance
Förster or fluorescence resonance energy transfer (FRET) technology ... provide a powerful tool for visualizing signaling molecules in live cells with high spatiotemporal resolution.
Evidence 2Source 2DOIPubMedprovenance
ET acceptor/sensitization properties which have been ascribed to Förster resonance energy transfer (FRET)...
Evidence 3Source 3DOIPubMedprovenance
Förster resonance energy transfer (FRET) is applied extensively in all fields of biological research and technology, generally as a 'nanoruler' with a dynamic range corresponding to the intramolecular and intermolecular distances characterizing the molecular structures that regulate cellular function.
Evidence 4Source 4DOIPubMedprovenance

Supporting Sources

Linked Claims

Claim 1mechanism summarysupports2017Source 1DOIPubMed

The MC form can act as an energy acceptor from fluorescent molecules during energy transfer processes under proper conditions.

Quoted textsource-backed
The MC form not only gives complexes with various inorganic particles, biological molecules, and organic chemicals but also acts as the energy acceptor (of energy from fluorescent molecules) during energy transfer processes that take place under proper conditions.
Claim 2mechanism interpretationsupports2016Source 3DOIPubMed

The data suggest that AuNC sensitization is not explained by classical FRET or NSET.

Quoted textsource-backed
Cumulatively, data suggests that AuNC sensitization is not by classical FRET or NSET...
Claim 3model fitsupports2016Source 3DOIPubMed

Classical FRET theory dramatically underestimated the observed energy transfer in these AuNC systems.

Quoted textsource-backed
FRET theory dramatically underestimated the observed energy transfer...
Claim 4utility summarysupports2016Source 2DOIPubMed

FRET technology and genetically encoded FRET biosensors are useful for visualizing signaling molecules in live cells with high spatiotemporal resolution.

Claim 5capability summarysupports2006Source 4DOIPubMed

FRET functions as a nanoruler over intramolecular and intermolecular distance ranges relevant to molecular structures that regulate cellular function.

Quoted textsource-backed
Förster resonance energy transfer (FRET) is applied extensively in all fields of biological research and technology, generally as a 'nanoruler' with a dynamic range corresponding to the intramolecular and intermolecular distances characterizing the molecular structures that regulate cellular function.