Directed Evolution of Fluorescent Genetically Encoded Biosensors: Innovative Approaches for Development and Optimization of Biosensors.
First-pass extracted concept
fluorescent genetically encoded biosensors
Aliases
fluorescent protein-based biosensors
Evidence Snippets
Supporting Sources
Linked Claims
Directed evolution offers a more efficient alternative than rational design for developing fluorescent genetically encoded biosensors.
Incorporating machine learning into directed evolution has the potential to enhance efficiency and reduce the cost of biosensor development.
Recent directed evolution approaches for biosensor development include optimizing domain fusions, sequence optimization, and new screening and selection systems.
Fluorescent protein-based biosensors are indispensable molecular tools in cell biology and biomedical research for non-invasive dynamic measurements of metabolite concentrations and other cellular signals.