First-pass extracted concept

GC-rich plasmid sequences

Candidate: concept label1 source documents3 linked claims

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Aliases

GC-rich sequences

Evidence Snippets

GC-rich sequences affect DNA replication, recombination and repair, as well as RNA transcription in vivo. Such sequences may also impede site-directed mutagenesis in vitro.

Evidence 1Source 1DOI PubMedprovenance

Supporting Sources

Source 1primary paper2025MEDDOI PubMed

1 evidence snippet 3 linked claims Claim 1 Claim 2 Claim 3

Linked Claims

Claim 1causal sequence feature effectsupports2025Source 1DOI PubMed

Either of the two GC-rich regions was sufficient to render the plasmid incompatible with P3a mutagenesis.

Quoted textsource-backed

deletion mutagenesis revealed that either of the two GC-rich regions is sufficient for rendering the plasmid incompatible with P3a mutagenesis

Claim 2mechanistic hypothesissupports2025Source 1DOI PubMed

GC-rich regions in the synthetic CAG promoter and KAT2B coding region may form G-quadruplexes and hinder plasmid denaturation during PCR.

Quoted textsource-backed

two GC-rich regions located within the synthetic CAG promoter and the KAT2B coding region may form guanine (G)-quadruplexes and hinder plasmid denaturation during PCR

Claim 3mechanistic interpretationsupports2025Source 1DOI PubMed

The findings support that G-quadruplex formation is one mechanism by which GC-rich sequences impede regular PCR-based mutagenesis methods.

Quoted textsource-backed

The findings support that G-quadruplex formation is one mechanism whereby such sequences impede regular PCR-based mutagenesis methods.