genetic circuit-enabled synthetic biosensors

Synthetic biosensors are presented as promising biosensing platforms for on-site, sustainable, affordable, and easy-to-use detection across environmental monitoring, disease diagnosis, food safety control, and bioproduction optimization.

Field deployment and real-world application of synthetic biosensors face challenges in sensitivity, specificity, speed, stability, and biosafety.

Design principles, enabling tools, and engineering strategies for creating high-performing synthetic biosensors include tuning dose-response characteristics such as detection limit, detection threshold, operating range, dynamic range, and leakiness.

Synthetic biosensors can be functionally extended by customizing signal-processing and output modules.

Genetic circuit-enabled synthetic biosensors in collaboration with materials science, electronic engineering, and artificial intelligence are expected to expand the application space of synthetic biology.