This source describes fluorescent-protein-based genetically encoded biosensors that report redox-related physiological parameters in live plant systems. They are presented as tools for accessing retrograde redox signalling in cells, tissues, and whole plants.
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genetically encoded redox biosensors
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Genetically encoded biosensors based on fluorescent proteins have been developed for a number of different redox-related physiological parameters and can be monitored in living cells, tissues, and even whole plants using a variety of instruments adapted to the respective resolution requirements, thus opening gateways to retrograde signalling in plant cells.
Genetically-encoded redox biosensors have become invaluable tools for monitoring cellular redox processes with high spatiotemporal resolution, coupling the presence of the redox-active analyte with a change in fluorescence signal that can be easily recorded.
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Fluorescent-protein-based genetically encoded biosensors have been developed for multiple redox-related physiological parameters and can be monitored in living cells, tissues, and whole plants.
Genetically encoded biosensors based on fluorescent proteins have been developed for a number of different redox-related physiological parameters and can be monitored in living cells, tissues, and even whole plants using a variety of instruments adapted to the respective resolution requirements
Genetically encoded biosensors can be combined with genetic and pharmacological approaches to extract meaningful information and dissect retrograde redox signalling systems in living plants.
It also outlines how biosensors can be used in combination with genetic and pharmacological approaches, to extract meaningful information and dissect the retrograde redox signalling systems in living plants.
The review classifies redox biosensors by the analytes they respond to and summarizes available fluorescence recording methods.
The review examines redox biosensor design, sensing mechanisms, and practical advantages and disadvantages.
Genetically encoded redox biosensors are useful for monitoring cellular redox processes with high spatiotemporal resolution by coupling analyte presence to a recordable fluorescence change.