GEVIs are optical reporters of membrane voltage with high spatial and temporal resolution. The abstract describes improved versions with higher brightness, sensitivity, and faster kinetics.
First-pass extracted concept
genetically encoded voltage indicators
Candidate: concept label6 source documents10 linked claims
Live refresh every 5sNext refresh in 5s
Aliases
GEVI, GEVIs
Extracted Explainers
What the tool is doing
Resources required
Use requires genetic targeting of the indicator to neural populations and optical measurement of the resulting signals.
The abstract supports only general requirements of genetic encoding and optical imaging, not specific hardware or construct details for this class.
What problem it solves
What it does not solve
Evidence Snippets
The primary sources of signal degradation ... are systematically explored across four major indicator classes: voltage-sensitive dyes (VSDs), genetically encoded voltage indicators (GEVIs), calcium-sensitive dyes (CSDs), and genetically encoded calcium indicators (GECIs).
Monitoring neuronal activation by a magnetogenetics approach can be facilitated by the co-expression of genetically-encoded voltage indicators (GEVI)
Voltage imaging using genetically encoded voltage indicators (GEVIs) has taken the field of neuroscience by storm in the past decade.
Genetically encoded voltage indicators report membrane voltage with high spatiotemporal resolution.
Probing the neural circuit dynamics underlying behaviour would benefit greatly from improved genetically encoded voltage indicators.
In particular, we take an in-depth look at the design of available GINA families with a particular focus on genetically encoded calcium indicators (GCaMPs), sensors probing synaptic activity, and genetically encoded voltage indicators.
Supporting Sources
Linked Claims
The review summarizes mitigation strategies for signal-quality limitations including hardware optimization, sensor choice, sample preparation, experimental design, post-processing, and computational correction methods.
The review classifies common mechanisms that compromise data quality into photon shot noise, device-related errors, and sample-related measurement errors.
Signal degradation in biological optical imaging of membrane voltage and calcium dynamics is organized in this review across four indicator classes: VSDs, GEVIs, CSDs, and GECIs.
The usefulness of voltage imaging with GEVIs depends critically on the kinetics of the indicator response to voltage.
Voltage imaging using genetically encoded voltage indicators can provide subcellular and network-level readouts of electrical dynamics.
Enhanced GEVIs can measure membrane voltage of neural populations at cellular resolution in vitro and in vivo at high speeds.
Quoted textsource-backed
Such capabilities have broadened the GEVIs' ability to measure membrane voltage of neural populations at cellular resolution in vitro and in vivo, all at high speeds.
High voltage fidelity and fast GEVI responses have revealed novel physiological phenomena in neuroscientific applications.
Quoted textsource-backed
The GEVIs' high voltage fidelity and fast response have revealed novel physiological phenomena in multiple neuroscientific applications.
Genetically encoded voltage indicators report membrane voltage with high spatiotemporal resolution.
Quoted textsource-backed
Genetically encoded voltage indicators report membrane voltage with high spatiotemporal resolution.
Recent improvements in GEVI brightness, sensitivity, and kinetics increased signal-to-noise performance by more than ten-fold and reduced response time to the sub-millisecond regime.
Quoted textsource-backed
Extensive recent efforts to improve the GEVIs' brightness, sensitivity, and kinetics have greatly increased the GEVIs' signal-to-noise performance over ten-fold and lowered their response time to the sub-millisecond regime.
The review focuses in depth on GCaMP-family calcium indicators, synaptic activity sensors, and genetically encoded voltage indicators as major GINA classes.
Quoted textsource-backed
we take an in-depth look at the design of available GINA families with a particular focus on genetically encoded calcium indicators (GCaMPs), sensors probing synaptic activity, and genetically encoded voltage indicators