First-pass extracted concept

geometry-matched strand-invasion window

Candidate: concept label1 source documents4 linked claims

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Extracted Explainers

What the tool is doing

This concept describes aligning Cas9 cut placement and donor geometry with the endogenous strand-invasion path during HDR.

Source 1DOI PubMed

Evidence Snippets

create a geometry-matched strand-invasion window

Evidence 1Source 1DOI PubMedprovenance

Supporting Sources

Source 1primary paper2026MEDDOI PubMed

1 evidence snippet 4 linked claims Claim 1 Claim 2 Claim 3

Linked Claims

Claim 1mechanistic insightsupports2026Source 1DOI PubMed

CRISPR-Cas9 knock-in efficiency is limited by geometric alignment between donor DNA and the endogenous strand-invasion path.

Claim 2mechanistic modelsupports2026Source 1DOI PubMed

Pairing fidelity between the resected chromosomal strand and donor homology arms governs knock-in outcomes.

Claim 3mechanistic observationsupports2026Source 1DOI PubMed

Chromatin immunoprecipitation-based profiling shows directional loading of the RAD51 homolog UvsC around Cas9-induced double-strand breaks, defining the spatial origin of strand invasion.

Claim 4mechanistic observationsupports2026Source 1DOI PubMed

In Aspergillus nidulans, integration drops sharply when the insertion site is offset from the strand-invasion entry point.