First-pass extracted concept

GPCR expression, stabilization, and crystallography

Candidate: concept label1 source documents5 linked claims

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Extracted Explainers

What the tool is doing

This concept groups the technological developments that the abstract says were required to obtain high-resolution GPCR structures. It functions as enabling methodology rather than a single named tool.

Source 1DOI PubMed

Resources required

The abstract supports that expression, stabilization, and crystallography capabilities were all needed, but does not specify exact platforms or reagents.

Source 1DOI PubMed

What problem it solves

It addresses the long-standing difficulty of obtaining high-resolution structures for GPCRs, including hard lipid-binding families.

Source 1DOI PubMed

What it does not solve

The abstract does not show that these developments alone solve receptor pharmacology, therapeutic design, or all family-specific structural challenges.

Source 1DOI PubMed

Evidence Snippets

More than 7 years after this initial discovery and following more than 20 years of technological developments in GPCR expression, stabilization, and crystallography, the high-resolution structure of the adrenaline binding β2 -adrenergic receptor, a ligand diffusible receptor, was discovered.

Evidence 1Source 1DOI PubMedprovenance

Supporting Sources

Source 1review2018Protein ScienceDOI PubMed

1 evidence snippet 5 linked claims Claim 1 Claim 2 Claim 3

Linked Claims

Claim 1enabling technology summarysupports2018Source 1DOI PubMed

High-resolution structure determination of the β2-adrenergic receptor followed more than 20 years of technological developments in GPCR expression, stabilization, and crystallography.

Quoted textsource-backed

following more than 20 years of technological developments in GPCR expression, stabilization, and crystallography, the high-resolution structure of the adrenaline binding β2 -adrenergic receptor ... was discovered

Claim 2field progress summarysupports2018Source 1DOI PubMed

High-resolution structures of more than 53 unique GPCRs have been determined.

Quoted textsource-backed

Since then, high-resolution structures of more than 53 unique GPCRs have been determined

Claim 3historical structural milestonesupports2018Source 1DOI PubMed

Bovine rhodopsin was the first GPCR with a solved crystal structure, reported in 2000.

Quoted textsource-backed

The first crystal structure of a G protein-coupled receptor (GPCR) was that of the bovine rhodopsin, solved in 2000

Claim 4mechanistic insight summarysupports2018Source 1DOI PubMed

The accumulation of high-resolution GPCR structures substantially improved understanding of ligand binding and ligand-mediated receptor activation mechanisms.

Quoted textsource-backed

leading to a significant improvement in our understanding of the basic mechanisms of ligand-binding and ligand-mediated receptor activation

Claim 5translational relevance summarysupports2018Source 1DOI PubMed

Sustained research into GPCR structural biology is important for development of new therapeutics targeting lipid receptors.

Quoted textsource-backed

highlighting the importance of sustained research into the structural biology of GPCRs for the development of new therapeutics targeting lipid receptors