Subsequent transcriptomic analysis was performed by integrating second-generation (Illumina) and third-generation (PacBio) sequencing technologies.
First-pass extracted concept
Integrated Illumina and PacBio transcriptomics
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Sequencing generated 77,930,985 bp of total transcript length, identifying 40,359 unique genes, of which 38,791 were annotated.
Quoted textsource-backed
Sequencing generated a total transcript length of 77,930,985 bp, identifying 40,359 unique genes, with 38,791 successfully annotated.
Under drought stress, sugarcane variety ZZ9 showed enrichment and upregulation of pathways related to photosynthesis, plant hormones, polysaccharide synthesis, and amino acid metabolism.
Quoted textsource-backed
Under drought stress, the variety ZZ9 exhibited significant enrichment and upregulation of metabolic pathways related to photosynthesis, plant hormones, polysaccharide synthesis, and amino acid metabolism.
Several transcription factor families including bHLH, bZIP, ERF, NAC, MYB, and GRAS were drought-inducible in the study.
Quoted textsource-backed
Several transcription factor families, including bHLH, bZIP, ERF, NAC, MYB, and GRAS, were drought-inducible.
The study integrated Illumina and PacBio transcriptomic sequencing to generate a comprehensive sugarcane transcriptome landscape under PEG6000-simulated drought stress.
Quoted textsource-backed
Subsequent transcriptomic analysis was performed by integrating second-generation (Illumina) and third-generation (PacBio) sequencing technologies. This approach yielded a comprehensive transcriptome landscape.