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Isl1-positive and Tbx2b-positive ring-shaped pacemaker region in zebrafish heart

Candidate: concept label1 source documents5 linked claims
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Aliases

Isl1-expressing ring-shaped structure around the venous pole, previously unidentified Isl1-positive and Tbx2b-positive region

Extracted Explainers

What the tool is doing

This source describes a ring-shaped Isl1-positive and Tbx2b-positive myocardial region at the venous pole of the zebrafish heart that contains pacemaker cells. The paper presents it as a structurally and molecularly distinguishable component of the cardiac conduction system.

Source 1DOIPubMed

Resources required

The abstract states that in vivo transgenic labeling was used to mark Isl1-positive cells, followed by isolation and electrophysiological characterization. It also references 3D reconstruction of gene expression patterns in embryonic and adult zebrafish hearts.

Source 1DOIPubMed

What problem it solves

It addresses the prior lack of an identified structural or molecularly distinguishable conduction-system component in lower vertebrates. The study uses this region to localize pacemaker function in adult zebrafish heart.

Source 1DOIPubMed

What it does not solve

The abstract does not define the full molecular program, standardized nomenclature, or all functional mechanisms of these pacemaker cells. It also does not describe broader engineering or therapeutic use.

Source 1DOIPubMed

Alternatives

The abstract contrasts this zebrafish pacemaker region with the mammalian sinus node and mammalian Tbx3- and Isl1-expressing sinus node progenitors.

Source 1DOIPubMed

Evidence Snippets

3D reconstructions of gene expression patterns in the embryonic and adult zebrafish heart led us to uncover a previously unidentified, Isl1-positive and Tbx2b-positive region in the myocardium at the junction of the sinus venosus and atrium. Through their long interconnecting cellular protrusions the identified Isl1-positive cells form a ring-shaped structure.
Evidence 1Source 1DOIPubMedprovenance

Supporting Sources

Linked Claims

Claim 1anatomical identificationsupports2012Source 1DOIPubMed

A previously unidentified Isl1-positive and Tbx2b-positive myocardial region exists at the junction of the sinus venosus and atrium in zebrafish heart.

Quoted textsource-backed
3D reconstructions of gene expression patterns in the embryonic and adult zebrafish heart led us to uncover a previously unidentified, Isl1-positive and Tbx2b-positive region in the myocardium at the junction of the sinus venosus and atrium.
Claim 2comparative biologysupports2012Source 1DOIPubMed

The identified zebrafish pacemaker structure is an evolutionarily conserved, structural and molecularly distinguishable component of the cardiac conduction system in a lower vertebrate.

Quoted textsource-backed
We have thereby identified an evolutionary conserved, structural and molecular distinguishable component of the cardiac conduction system in a lower vertebrate.
Claim 3functional assignmentsupports2012Source 1DOIPubMed

Isl1-expressing cells organized as a ring-shaped structure around the venous pole hold the pacemaker function in the adult zebrafish heart.

Quoted textsource-backed
In conclusion we demonstrate that Isl1-expressing cells, organized as a ring-shaped structure around the venous pole, hold the pacemaker function in the adult zebrafish heart.
Claim 4functional characterizationsupports2012Source 1DOIPubMed

In vivo transgenic labeling of Isl1-positive cells enabled their isolation and electrophysiological characterization, which revealed unique pacemaker activity.

Quoted textsource-backed
In vivo labeling of the Isl1-positive cells by transgenic technology allowed their isolation and electrophysiological characterization, revealing their unique pacemaker activity.
Claim 5genetic perturbation phenotypesupports2012Source 1DOIPubMed

Zebrafish embryos lacking Isl1 display heart rate defects related to pacemaker dysfunction.

Quoted textsource-backed
Here we show that zebrafish embryos lacking the LIM/homeodomain-containing transcription factor Isl1 display heart rate defects related to pacemaker dysfunction.