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lens-based fluorescence nanoscopy

Candidate: concept label1 source documents4 linked claims
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Aliases

fluorescence nanoscopy, nanoscopy, super-resolution microscopy

Extracted Explainers

What the tool is doing

Lens-based fluorescence nanoscopy comprises far-field super-resolution imaging methods that separate nearby features by controlling fluorophore emission states over time. The review frames it as a move beyond diffraction-limited fluorescence microscopy.

Source 1DOIPubMed

Resources required

These methods require fluorescent labels and optical setups capable of exploiting transient differences in fluorophore emission states. Some variants additionally require specialized fluorophore classes.

Source 1DOIPubMed

What problem it solves

It addresses the inability of conventional optical microscopy to resolve structural details much finer than about half the wavelength of visible light.

Source 1DOIPubMed

What it does not solve

The abstract does not support a single universally optimal nanoscopy method, and it notes that variants differ in advantages, disadvantages, and fluorophore requirements.

Source 1DOIPubMed

Alternatives

The review contrasts nanoscopy with conventional far-field fluorescence microscopy limited by diffraction.

Source 1DOIPubMed

Evidence Snippets

The surpassing of this resolution limit in far-field microscopy is currently one of the most momentous developments for studying the living cell, as the move from microscopy to super-resolution microscopy or 'nanoscopy' offers opportunities to study problems in biophysical and biomedical research at a new level of detail.
Evidence 1Source 1DOIPubMedprovenance

Supporting Sources

Linked Claims

Claim 1application scopesupports2015Source 1DOIPubMed

Similar to conventional far-field microscopy, nanoscopy can be utilized for dynamical, multi-color and three-dimensional imaging of fixed and live cells, tissues or organisms.

Quoted textsource-backed
Similar to conventional far-field microscopy, nanoscopy can be utilized for dynamical, multi-color and three-dimensional imaging of fixed and live cells, tissues or organisms.
Claim 2capabilitysupports2015Source 1DOIPubMed

Fluorescence nanoscopy variants can in principle reach molecular spatial resolution.

Quoted textsource-backed
Each of the variants can in principle reach molecular spatial resolution and has its own advantages and disadvantages.
Claim 3principlesupports2015Source 1DOIPubMed

Existing fluorescence nanoscopy variants separate neighboring features by transiently preparing fluorescent molecules in states of different emission characteristics, usually on and off states that cause adjacent molecules to emit sequentially in time.

Quoted textsource-backed
All the existing nanoscopy variants separate neighboring features by transiently preparing their fluorescent molecules in states of different emission characteristics in order to make the features discernible. Usually these are fluorescent 'on' and 'off' states causing the adjacent molecules to emit sequentially in time.
Claim 4requirementsupports2015Source 1DOIPubMed

Some nanoscopy variants require specific fluorophore transitions and states found only in certain fluorophore subfamilies such as photoswitchable fluorophores, whereas other variants can be realized with standard fluorescent labels.

Quoted textsource-backed
Some require specific transitions and states that can be found only in certain fluorophore subfamilies, such as photoswitchable fluorophores, while other variants can be realized with standard fluorescent labels.