Super-resolution optical imaging based on the switching and localization of individual fluorescent molecules [photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), etc.] has evolved remarkably over the last decade.
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localization-based super-resolution microscopy
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super-resolution optical imaging based on the switching and localization of individual fluorescent molecules
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Analytical methods are an important part of interpreting localization-based super-resolution data.
Quoted textsource-backed
and address some of the more common analytical methods used with this data.
Localization-based super-resolution optical imaging has evolved from a technology-driven effort to push imaging limits into a tool of biological discovery.
Quoted textsource-backed
Super-resolution optical imaging based on the switching and localization of individual fluorescent molecules [photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), etc.] has evolved remarkably over the last decade. Originally driven by pushing technological limits, it has become a tool of biological discovery.
The field has shifted from prioritizing impressive images of well-studied structures toward quantitative, reliable data that can test unresolved biological hypotheses.
Quoted textsource-backed
The initial demand for impressive pictures showing well-studied biological structures has been replaced by a need for quantitative, reliable data providing dependable evidence for specific unresolved biological hypotheses.
Defining resolution in localization-based super-resolution microscopy is a complex problem.
Quoted textsource-backed
In this context, we consider the complex topic of defining resolution for this imaging modality