First-pass extracted concept

multiplexed CRISPR-Cas

Candidate: concept label1 source documents4 linked claims

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Aliases

CRISPR-based editing technologies for multiple targets, multiplexed genome editing

Extracted Explainers

What the tool is doing

Multiplexed CRISPR-Cas refers to using CRISPR-Cas to target multiple sites simultaneously for genome engineering. The abstract frames it as a flexible multi-target editing approach.

Source 1DOI PubMed

Resources required

The abstract states that CRISPR-Cas is modulated by a short guide RNA, implying guide RNA design and a CRISPR-Cas effector are required.

Source 1DOI PubMed

What problem it solves

It solves the need to perturb multiple targets at once for applications such as large deletions, structural variation generation, epigenetic editing, and selective cell killing.

Source 1DOI PubMed

Alternatives

The abstract contrasts CRISPR-Cas with other genome editing agents, but does not name specific alternatives.

Source 1DOI PubMed

Evidence Snippets

Due to its simplicity, CRISPR-Cas is recognized as the best candidate for multiplexed genome editing.

Evidence 1Source 1DOI PubMedprovenance

Supporting Sources

Source 1primary paper2025MEDDOI PubMed

1 evidence snippet 4 linked claims Claim 1 Claim 2 Claim 3

Linked Claims

Claim 1application capabilitysupports2025Source 1DOI PubMed

CRISPR-Cas can induce complex structural variations including inversions, translocations, and duplications.

Claim 2application capabilitysupports2025Source 1DOI PubMed

Multiplexed CRISPR targeting can enable killing of specific cell types by accumulating stress from simultaneous DNA damages.

Claim 3application capabilitysupports2025Source 1DOI PubMed

Simultaneous targeting with multiplexed CRISPR-Cas can enable efficient gene knockout with large deletions.

Claim 4comparative suitabilitysupports2025Source 1DOI PubMed

CRISPR-Cas is recognized as a strong candidate for multiplexed genome editing because it is modulated by a short guide RNA and is simple to use for simultaneous targeting.