Conformational changes in oat phytochrome A (phy) in solution after photoexcitation of the red-absorbing form (Pr) were studied in time-domain by the pulsed laser-induced transient grating technique.
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oat phytochrome A
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phy, phyA
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For intact phy, diffusion first decreases within 1 ms after photoexcitation and then increases with a time constant of 100 ms to the Pfr value.
Quoted textsource-backed
D of Pr of intact phy (4.1 x 10(-11) m(2) s(-1)) first decreases upon photoexcitation to 0.89 x 10(-11) m(2) s(-1) within 1 ms and then gradually increases with a time constant of 100 ms to the value of Pfr, 1.7 x 10(-11) m(2) s(-1).
For large phy, the diffusion coefficient of the Pfr form is markedly lower than that of the Pr form.
Quoted textsource-backed
the diffusion coefficient (D) of far-red-absorbing form (Pfr) of large phy (1.3 x 10(-11) m(2) s(-1)) is markedly reduced compared with that of Pr (5.8 x 10(-11) m(2) s(-1))
The reduced diffusion coefficient of large phy Pfr relative to Pr indicates that Pfr has a significantly changed conformation with increased intermolecular interaction with water molecules.
Quoted textsource-backed
This large reduction indicates that the conformation of Pfr is significantly changed from that of Pr, so that the intermolecular interaction with water molecules increases. This change completes within 1 ms after the photoexcitation.
The slower approximately 100 ms phase in intact phy suggests a conformational change in the N-terminal region that decreases intermolecular interaction with water after a global change in the large phy region.
Quoted textsource-backed
This slower phase suggests that the conformation of the N-terminal region changes with 100 ms to decrease the intermolecular interaction with water after a global change in the large phy region.
Pulsed laser-induced transient grating was used to study time-domain conformational changes in oat phytochrome A in solution after photoexcitation of the Pr form.
Quoted textsource-backed
Conformational changes in oat phytochrome A (phy) in solution after photoexcitation of the red-absorbing form (Pr) were studied in time-domain by the pulsed laser-induced transient grating technique.
The increase in diffusion was interpreted as alpha-helix formation in the Pfr form from a random coil structure in the Pr form.
Quoted textsource-backed
The increase of D was interpreted in terms of alpha-helix formation in the Pfr form from the random coil structure in the Pr form.