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optogenetic reporters

Candidate: concept label2 source documents7 linked claims
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Extracted Explainers

What the tool is doing

Optogenetic reporters are genetically encoded light-sensitive proteins used for optical measurement of cellular activity. The abstract specifically notes voltage- and calcium-sensitive indicators in zebrafish heart studies.

Source 1DOIPubMed

Resources required

Their use requires reporter expression and fluorescence imaging methods. The abstract mentions increasingly sophisticated fluorescence imaging approaches as relevant to this area.

Source 1DOIPubMed

What problem it solves

They provide optical readouts of cardiac activity in zebrafish. This supports investigation of heart electrophysiology in vivo and in situ.

Source 1DOIPubMed

What it does not solve

The abstract does not specify whether reporters alone provide causal control of cardiac function, since that role is associated with actuators. It also does not detail reporter-specific limitations such as signal quality or kinetics.

Source 1DOIPubMed

Alternatives

The abstract contrasts reporters with actuators, which manipulate rather than measure activity. No other sensing modalities are explicitly named in the provided text.

Source 1DOIPubMed

Evidence Snippets

Zebrafish were also adopted early as an experimental model for the use of optogenetic reporters, including genetically encoded voltage- and calcium-sensitive indicators.
Evidence 1Source 1DOIPubMedprovenance
These optogenetic reporters are powerful tools for live-cell microscopy and quantitative analysis at the subcellular level.
Evidence 2Source 2DOIPubMedprovenance

Supporting Sources

Linked Claims

Claim 1future potentialsupports2021Source 1DOIPubMed

Increasingly sophisticated fluorescence imaging and spatially resolved light stimulation methods make zebrafish a model with unrealized potential for cardiac optogenetic studies.

Quoted textsource-backed
With the advent of increasingly sophisticated fluorescence imaging approaches and methods for spatially-resolved light stimulation in the heart, the zebrafish represents an experimental model with unrealized potential for cardiac optogenetic studies.
Claim 2model advantagesupports2021Source 1DOIPubMed

For cardiac optogenetic studies, zebrafish offer whole-heart in vivo visualization and interrogation in transparent externally developing embryos, and the small adult heart enables in situ cell-specific observation and control not possible in mammals.

Quoted textsource-backed
For optogenetic studies, zebrafish provide additional advantages, as the whole zebrafish heart can be visualized and interrogated in vivo in the transparent, externally developing embryo, and the relatively small adult heart allows for in situ cell-specific observation and control not possible in mammals.
Claim 3model advantagesupports2021Source 1DOIPubMed

Zebrafish combine advantages of integrative and reduced experimental models, have genetic and functional cardiac similarities to mammals, can be genetically modified, recapitulate cardiac diseases, and allow high-throughput investigations.

Quoted textsource-backed
Beyond optogenetic studies, zebrafish are becoming an increasingly important tool for cardiac research, as they combine many of the advantages of integrative and reduced experimental models. The zebrafish has striking genetic and functional cardiac similarities to that of mammals, its genome is fully sequenced and can be modified using standard techniques, it has been used to recapitulate a variety of cardiac diseases, and it allows for high-throughput investigations.
Claim 4review summarysupports2021Source 1DOIPubMed

Optogenetics is a powerful experimental technique for optical measurement and manipulation of cellular activity using genetically encoded light-sensitive reporters and actuators.

Quoted textsource-backed
Optogenetics, involving the optical measurement and manipulation of cellular activity with genetically encoded light-sensitive proteins ("reporters" and "actuators"), is a powerful experimental technique for probing (patho-)physiological function.
Claim 5review summarysupports2021Source 1DOIPubMed

Zebrafish were adopted early for cardiac optogenetic reporters including genetically encoded voltage-sensitive and calcium-sensitive indicators.

Quoted textsource-backed
Zebrafish were also adopted early as an experimental model for the use of optogenetic reporters, including genetically encoded voltage- and calcium-sensitive indicators.
Claim 6capabilitysupports2012Source 2DOIPubMed

Optogenetic reporters are powerful tools for live-cell microscopy and quantitative analysis at the subcellular level.

Quoted textsource-backed
These optogenetic reporters are powerful tools for live-cell microscopy and quantitative analysis at the subcellular level.
Claim 7combined use casesupports2012Source 2DOIPubMed

Optogenetic actuators and reporters can be combined for simultaneous control and imaging of cell and tissue physiology.

Quoted textsource-backed
the combined use of various optogenetic actuators and reporters for simultaneously controlling and imaging the physiology of cells and tissues