First-pass extracted concept

optogenetic sensors

Candidate: concept label2 source documents6 linked claims

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Extracted Explainers

What the tool is doing

This review frames optogenetic sensors as light-emitting tools for monitoring neuronal activity in living animals. The abstract specifically includes genetically encoded calcium indicators and voltage sensors within this class.

Source 2DOI PubMed

Resources required

Use in zebrafish requires strategies for high transgene expression in defined neuronal populations and optical methods for sample illumination. The abstract also implies a living, intact animal imaging setup.

Source 2DOI PubMed

What problem it solves

These tools help investigators observe neuronal circuit function with minimal perturbation and high spatiotemporal resolution.

Source 2DOI PubMed

What it does not solve

The abstract does not specify which individual sensor designs, performance limits, or failure modes are best for particular experiments.

Source 2DOI PubMed

Alternatives

The abstract contrasts sensors with optogenetic actuators, which control rather than monitor activity.

Source 2DOI PubMed

Evidence Snippets

development of optogenetic effectors and sensors

Evidence 1Source 1DOI PubMedprovenance

In this review, we describe some of the most recent advances in the development and applications of optogenetic sensors (i.e., genetically encoded calcium indicators and voltage sensors)

Evidence 2Source 2DOI PubMedprovenance

Supporting Sources

Source 1review2014Frontiers in PhysiologyDOI PubMed

1 evidence snippet 2 linked claims Claim 1 Claim 2

Source 2review2011Reviews in the NeurosciencesDOI PubMed

1 evidence snippet 4 linked claims Claim 1 Claim 2 Claim 3

Linked Claims

Claim 1bottlenecksupports2014Source 1DOI PubMed

A major barrier to wider use of optogenetic imaging tools is the lack of readily available genetic reagents that can be easily combined to probe complex biological processes.

Claim 2field progresssupports2014Source 1DOI PubMed

The review states that significant progress has been made in the development of optogenetic effectors and sensors for molecular-level study of complex biological signaling in mammals.

Claim 3implementation requirementsupports2011Source 2DOI PubMed

Implementing optogenetics in zebrafish requires strategies for high transgene expression in defined neuronal populations and optical methods that permit precise spatiotemporal control of illumination.

Quoted textsource-backed

We also describe crucial technical aspects to implement optogenetics in zebrafish including strategies to drive a high level of transgene expression in defined neuronal populations, and recent optical advances that allow the precise spatiotemporal control of sample illumination.

Claim 4model system suitabilitysupports2011Source 2DOI PubMed

Zebrafish neuronal circuits are presented as an ideal system for optogenetic analysis because of transparency, relatively small size, and amenability to genetic manipulation.

Quoted textsource-backed

Zebrafish neuronal circuits represent an ideal system to apply an optogenetic based analysis owing to its transparency, relatively small size and amenability to genetic manipulation.

Claim 5review scope summarysupports2011Source 2DOI PubMed

Optogenetics provides a combined sensor-and-actuator toolkit for monitoring and controlling neuronal activity in intact living animals with minimal perturbation and high spatiotemporal resolution.

Quoted textsource-backed

With a combination of light emitting 'sensors' and light activated 'actuators', we can monitor and control neuronal activity with minimal perturbation and unprecedented spatiotemporal resolution.

Claim 6tool class coveragesupports2011Source 2DOI PubMed

The review covers recent advances in optogenetic sensors including genetically encoded calcium indicators and voltage sensors, and actuators including light-activated ion channels and ion pumps.

Quoted textsource-backed

we describe some of the most recent advances in the development and applications of optogenetic sensors (i.e., genetically encoded calcium indicators and voltage sensors) and actuators (i.e., light activated ion channels and ion pumps).