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PFK1 deletion in Saccharomyces cerevisiae

Candidate: concept label1 source documents4 linked claims
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Aliases

PFK1 deletion, pfk1Δ strain

Extracted Explainers

What the tool is doing

PFK1 deletion perturbs yeast central carbon metabolism and serves as a direct comparison to PFK2 deletion. The abstract reports that its effects are less severe than those of pfk2Δ.

Source 1DOIPubMed

Resources required

This comparison requires a pfk1Δ yeast strain and phenotyping of growth and metabolite outputs. The abstract does not provide protocol details.

Source 1DOIPubMed

What problem it solves

It helps separate the distinct roles of the two phosphofructokinase subunits in S. cerevisiae metabolism. The study uses it as the main comparator against pfk2Δ.

Source 1DOIPubMed

What it does not solve

It does not produce the stronger respiratory shift or the reported free-fatty-acid improvement attributed to PFK2 deletion. The abstract does not present it as a beneficial engineering intervention.

Source 1DOIPubMed

Alternatives

The main alternative discussed in the source is PFK2 deletion, which produced stronger defects and stronger metabolic rewiring.

Source 1DOIPubMed

Evidence Snippets

Here, we examined the metabolic consequences of deleting PFK1 or PFK2... The pfk2Δ strain exhibited more severe defects than pfk1Δ.
Evidence 1Source 1DOIPubMedprovenance

Supporting Sources

Linked Claims

Claim 1comparative phenotypesupports2026Source 1DOIPubMed

In Saccharomyces cerevisiae, PFK2 deletion causes more severe central carbon metabolism defects than PFK1 deletion.

Quoted textsource-backed
The pfk2Δ strain exhibited more severe defects than pfk1Δ.
Claim 2growth effectsupports2026Source 1DOIPubMed

PFK2 deletion reduces maximum specific growth rate more strongly than PFK1 deletion in Saccharomyces cerevisiae.

Quoted textsource-backed
Its maximum specific growth rate was reduced by approximately 54 % in pfk2Δ and by about 15 % in pfk1Δ, both relative to the reference strain.
Claim 3metabolite accumulation effectsupports2026Source 1DOIPubMed

Both PFK1 and PFK2 deletion increase acetate accumulation in Saccharomyces cerevisiae, with a larger increase in pfk2Δ.

Quoted textsource-backed
Both deletion strains accumulated higher acetate levels compared to the reference strain, increasing by 25.4 % in the pfk1Δ strain and 82 % in the pfk2Δ strain.
Claim 4metabolite production effectsupports2026Source 1DOIPubMed

PFK2 deletion decreases ethanol production more strongly than PFK1 deletion in Saccharomyces cerevisiae.

Quoted textsource-backed
Ethanol production decreased by 36 % and 82 % in pfk1Δ strain and pfk2Δ strain, respectively, relative to the reference strain.