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PFK2 deletion in Saccharomyces cerevisiae

Candidate: concept label1 source documents8 linked claims
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Aliases

PFK2 deletion, pfk2Δ strain

Extracted Explainers

What the tool is doing

PFK2 deletion in S. cerevisiae shifts central carbon metabolism away from fermentation and toward respiration and biosynthesis. The abstract reports stronger physiological and flux changes for pfk2Δ than for pfk1Δ.

Source 1DOIPubMed

Resources required

This perturbation requires constructing a pfk2Δ yeast strain and measuring growth, metabolite production, flux balance analysis, transcriptomics, or product titers. The abstract does not specify the exact engineering or assay protocols.

Source 1DOIPubMed

What problem it solves

It provides a metabolic engineering lever to enhance acetyl-CoA-derived product formation, including free fatty acids. The paper frames PFK2 as a key regulatory node for redirecting carbon flux.

Source 1DOIPubMed

What it does not solve

It does not preserve normal growth or ethanol production, because pfk2Δ showed major growth and fermentation defects. The abstract does not show that this perturbation is broadly optimal across products or conditions.

Source 1DOIPubMed

Alternatives

The paper directly contrasts PFK2 deletion with PFK1 deletion. PFK1 deletion caused milder defects and less pronounced metabolic rewiring than PFK2 deletion.

Source 1DOIPubMed

Evidence Snippets

The pfk2Δ strain exhibited more severe defects than pfk1Δ... deletion of PFK2 enhanced acetyl-CoA-derived product formation.
Evidence 1Source 1DOIPubMedprovenance

Supporting Sources

Linked Claims

Claim 1comparative phenotypesupports2026Source 1DOIPubMed

In Saccharomyces cerevisiae, PFK2 deletion causes more severe central carbon metabolism defects than PFK1 deletion.

Quoted textsource-backed
The pfk2Δ strain exhibited more severe defects than pfk1Δ.
Claim 2flux rewiringsupports2026Source 1DOIPubMed

Flux balance analysis indicates that PFK2 deletion increases carbon flux to the TCA cycle and elevates respiration-associated carbon flux relative to PFK1 deletion.

Quoted textsource-backed
Flux balance analysis (FBA) revealed a markedly increased carbon flux to the tricarboxylic acid cycle (TCA) in the pfk2Δ strain, with respiration-associated carbon flux elevated 1.5-fold compared to the pfk1Δ strain.
Claim 3growth effectsupports2026Source 1DOIPubMed

PFK2 deletion reduces maximum specific growth rate more strongly than PFK1 deletion in Saccharomyces cerevisiae.

Quoted textsource-backed
Its maximum specific growth rate was reduced by approximately 54 % in pfk2Δ and by about 15 % in pfk1Δ, both relative to the reference strain.
Claim 4metabolite accumulation effectsupports2026Source 1DOIPubMed

Both PFK1 and PFK2 deletion increase acetate accumulation in Saccharomyces cerevisiae, with a larger increase in pfk2Δ.

Quoted textsource-backed
Both deletion strains accumulated higher acetate levels compared to the reference strain, increasing by 25.4 % in the pfk1Δ strain and 82 % in the pfk2Δ strain.
Claim 5metabolite production effectsupports2026Source 1DOIPubMed

PFK2 deletion decreases ethanol production more strongly than PFK1 deletion in Saccharomyces cerevisiae.

Quoted textsource-backed
Ethanol production decreased by 36 % and 82 % in pfk1Δ strain and pfk2Δ strain, respectively, relative to the reference strain.
Claim 6product titer improvementsupports2026Source 1DOIPubMed

PFK2 deletion enhances acetyl-CoA-derived product formation and increases free fatty acid titer in Saccharomyces cerevisiae.

Quoted textsource-backed
Deletion of PFK2 enhanced acetyl-CoA-derived product formation, with free fatty acid (FFA) titers increasing from 412 mg L^-1 to 517 mg L^-1 (a 33.3 % increase).
Claim 7regulatory rolesupports2026Source 1DOIPubMed

PFK2 functions as a key regulatory node that redirects carbon flux from fermentation toward respiration and biosynthesis in Saccharomyces cerevisiae.

Quoted textsource-backed
These findings establish PFK2 as a key regulatory node redirecting carbon flux from fermentation toward respiration and biosynthesis.
Claim 8transcriptomic state changesupports2026Source 1DOIPubMed

Transcriptomic profiling shows upregulation of respiration-related genes in the PFK2 deletion strain relative to the reference strain.

Quoted textsource-backed
Transcriptomic profiling showed significant upregulation of respiration-related genes in the pfk2Δ strain compared to the reference strain.