A key assumption of the imaging is that the fluorophores are independent, though this is invalidated in the presence of photodestruction.
First-pass extracted concept
photodestruction
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Linked Claims
Photodestruction-related SOFI images can exhibit less noise and may be misinterpreted as more visually pleasing or informative.
Quoted textsource-backed
This additional signal is strong and the resulting images typically exhibit less noise. Accordingly, these images may be mis-interpreted as being more visually pleasing or more informative.
Photodestruction produces an additional SOFI signal that is not easily interpretable as sample structure.
Quoted textsource-backed
We find that photodestruction gives rise to an additional signal that does not present an easily interpretable view of the sample structure.
The authors identified two approaches that can correct even strong photodestruction, and one can be implemented directly in SOFI calculation software.
Quoted textsource-backed
We identify two approaches that can correct for the presence of even strong photodestruction, one of which can be implemented directly in the SOFI calculation software.
SOFI assumes independent fluorophores, and photodestruction invalidates this assumption.
Quoted textsource-backed
A key assumption of the imaging is that the fluorophores are independent, though this is invalidated in the presence of photodestruction.
The authors developed a procedure to robustly estimate how much a SOFI experiment is affected by photodestruction.
Quoted textsource-backed
To address this uncertainty, we develop a procedure that can robustly estimate to what extent any particular experiment is affected by photodestruction.