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photoswitchable fluorescent proteins

Candidate: concept label4 source documents6 linked claims
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Extracted Explainers

What the tool is doing

This is the main reviewed class of fluorescent proteins whose optical state can be changed by light. The review scope emphasizes their chemistry, mechanisms, and applications.

Source 1DOIPubMed

Photoswitchable fluorescent proteins are fluorescent probes that can switch between two light-controlled states. The abstract presents them as enabling reagents for several diffraction-unlimited imaging methods.

Source 2DOIPubMed

Resources required

They must be used with microscopy techniques that exploit photoswitching properties and with appropriate optical control of illumination.

Source 2DOIPubMed

What problem it solves

They solve the need for switchable fluorescent probes in superresolution imaging workflows that go beyond the diffraction limit.

Source 2DOIPubMed

What it does not solve

The abstract does not show that photoswitchable fluorescent proteins alone are sufficient for superresolution, because the methods also depend on altered optical setups or post-imaging analysis.

Source 2DOIPubMed

Alternatives

The abstract contrasts these probes with other contributors to superresolution performance, including altered optical setups, unique fluorescent probes more generally, and post-imaging analysis.

Source 2DOIPubMed

Evidence Snippets

Title: "Photoswitchable fluorescent proteins: ten years of colorful chemistry and exciting applications"; web research summary states the review centers on reversibly photoswitchable fluorescent proteins (RSFPs), their switching mechanisms, and applications.
Evidence 1Source 1DOIPubMedprovenance
Many of these techniques also depend strictly on the use of unique fluorescent proteins (FPs) with special photoswitching properties. These photoswitchable FPs are capable of switching between two states in response to light.
Evidence 2Source 2DOIPubMedprovenance
Some of them possess kindling property, some are photoactivatable, and some are photoswitchable.
Evidence 3Source 3DOIPubMedprovenance
The recent demonstration and utilization of fluorescent proteins whose fluorescence can be switched on and off has greatly expanded the toolkit of molecular and cell biology.
Evidence 4Source 4DOIPubMedprovenance

Supporting Sources

Linked Claims

Claim 1applicationsupports2013Source 2DOIPubMed

PALM, STORM, RESOLFT, and saturated structured illumination microscopy exploit fluorescent protein switching properties to achieve superior spatial resolution.

Quoted textsource-backed
All localization precision and patterned illumination techniques-such as photo-activation localization microscopy, stochastic optical reconstruction microscopy, reversible saturable optically linear transitions, and saturated structured illumination microscopy-take advantage of these inherent switching properties to achieve superior spatial resolution.
Claim 2application associationsupports2013Source 1DOIPubMed

Photoswitchable fluorescent proteins are associated with superresolution imaging applications.

Quoted textsource-backed
PubMed abstract indicates the review centers on reversibly photoswitchable fluorescent proteins (RSFPs), their switching mechanisms, and applications in superresolution imaging...
Claim 3mechanismsupports2013Source 2DOIPubMed

Photoswitchable fluorescent proteins can switch between two states in response to light.

Quoted textsource-backed
These photoswitchable FPs are capable of switching between two states in response to light.
Claim 4mechanism requirementsupports2013Source 2DOIPubMed

Many superresolution microscopy techniques depend strictly on fluorescent proteins with special photoswitching properties.

Quoted textsource-backed
Many of these techniques also depend strictly on the use of unique fluorescent proteins (FPs) with special photoswitching properties.
Claim 5review scopesupports2013Source 2DOIPubMed

The review analyzes positive and negative aspects of photoswitchable fluorescent proteins and suggests suitable fluorescent proteins for superresolution imaging.

Quoted textsource-backed
This review provides extensive analysis of the positive and negative aspects of photoswitchable FPs, highlighting their application in diffraction-unlimited imaging and suggesting the most suitable fluorescent proteins for superresolution imaging.
Claim 6photochemical classsupports2008Source 3DOIPubMed

Within GFP-like proteins, some members are kindling, some are photoactivatable, and some are photoswitchable.

Quoted textsource-backed
Some of them possess kindling property, some are photoactivatable, and some are photoswitchable.