Photoswitches are fluorophores used in microscopy that can be selectively switched between fluorescent and nonfluorescent states. The abstract presents them as key enabling molecules for subdiffraction-resolution imaging and molecular quantification.
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photoswitches
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photoswitchable fluorophores, reversible photoswitches
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chemists have been fascinated by photosensitive molecules capable of switching between isomeric forms, known as photoswitches.
Here we review recent progress in subdiffraction‐resolution fluorescence imaging microscopy using various photoswitchable fluorophores and strategies. Special emphasis will be placed on the design and development of photoswitches and the requirements photoswitches have to fulfill for successful use in photoswitching microscopy.
Optical control elements can be classified according to their molecular reversibility as non-reversible phototriggers where light breaks a chemical bond (e.g. caged ligands) and as photoswitches that reversibly photoisomerize.
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Linked Claims
Biological modulation by photoswitchable peptides is driven by structural changes associated with incorporated photoswitches.
Photoswitches are key molecules for subdiffraction-resolution fluorescence imaging microscopy.
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Here we review recent progress in subdiffraction‐resolution fluorescence imaging microscopy using various photoswitchable fluorophores and strategies.
Photoswitches can be used for molecular quantification, including determining densities and absolute numbers of proteins in specific subcellular compartments.
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Moreover, we demonstrate how photoswitches can be used advantageously for molecular quantification, i.e. the determination of densities and absolute numbers of proteins located in specific subcellular compartments
Photoswitches have requirements that must be fulfilled for successful use in photoswitching microscopy.
Quoted textsource-backed
Special emphasis will be placed on the design and development of photoswitches and the requirements photoswitches have to fulfill for successful use in photoswitching microscopy.
Subdiffraction-resolution fluorescence imaging techniques discussed in the paper are based on selective switching of fluorophores between fluorescent and nonfluorescent states.
Quoted textsource-backed
They are all based on the selective switching of fluorophores between a fluorescent and a nonfluorescent state and are therefore generalized under the denotation “Photoswitching Microscopy”.
Combining photoswitched manipulation with fluorescence detection of cell signaling has enabled non-invasive all-optical experiments on cell and tissue function in vitro and in vivo.
Ion channels have been one of the principal protein targets of photoswitched manipulation.
Optical control elements can be classified by molecular reversibility into non-reversible phototriggers and reversibly photoisomerizing photoswitches.
Optical manipulation of channels has provided insights into the mechanism of channel function.
Methods to manipulate proteins with light have produced major advances in recent years.