The paper describes selective synthesis of pigment-binding proteins in toluene-treated Rhodopseudomonas capsulata cells and in cell-free translation systems using isolated polysomes.
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pigment-binding protein synthesis in toluene-treated cells and cell-free systems
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Synthesis of pigment-binding polypeptides depends on concomitant synthesis of tetrapyrroles in toluene-treated cells.
The in vitro synthesized Mr-10,000 polypeptide is insoluble in chloroform/methanol but becomes organic-solvent-soluble after detergent denaturation in the presence of isolated membrane, indicating alternative conformations.
The findings indicate that the Mr-10,000 polypeptide may enter the lipid bilayer by a membrane-triggered conformational change.
Pigment-binding polypeptides of the light-harvesting complexes are encoded by messenger RNA of extreme longevity.
Pigment-binding protein of Rhodopseudomonas capsulata can be selectively synthesized in toluene-treated cells and in homologous and heterologous cell-free translation systems by isolated polysomes.
The Mr-28,000 reaction-center polypeptide and the Mr-10,000 light-harvesting complex II pigment-binding polypeptide are synthesized by free water-soluble polysomes without a cleavable leader or signal peptide.