This targeting strategy places ReaChR in retinal ganglion cells. The abstract shows that it can restore visual responses, but with less diverse and less reproducible coding than ON bipolar-cell targeting.
First-pass extracted concept
retinal ganglion cell targeting
Candidate: concept label3 source documents11 linked claims
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Aliases
Brn3c-expressing cells, RGCs, RGC targeting
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Evidence Snippets
retinal ganglion cells (RGCs) ... express the opsin ReaChR in ... Brn3c-expressing cells
retinal ganglion cells (RGCs)
multielectrode array recordings of retinal explants revealed robust and uniform light-evoked firing when LiGluR-MAG0(460) was targeted to RGCs
Supporting Sources
Linked Claims
Compared with ON bipolar-cell targeting, retinal ganglion-cell targeting of ReaChR decreased response reproducibility and produced more stereotyped responses with reduced diversity in response polarity, contrast sensitivity, and temporal frequency tuning.
ReaChR expression targeted to ON bipolar cells and retinal ganglion cells produced equivalent response sensitivity and contrast encoding across different background irradiances in retinally degenerate mice.
In visually intact retinas, retinal ganglion-cell-targeted ReaChR expression disrupted visual feature selectivity of individual retinal ganglion cells.
Both ON bipolar-cell and retinal ganglion-cell targeting strategies restored visual responses with high fidelity, but ON bipolar-cell targeting produced a richer visual code closer to wild-type mice.
Compared with ON bipolar cell targeting, retinal ganglion cell targeting decreased response reproducibility and produced more stereotyped responses with reduced diversity in response polarity, contrast sensitivity, and temporal frequency tuning.
Quoted textsource-backed
Compared to ON BCs, targeting RGCs decreased response reproducibility and resulted in more stereotyped responses with reduced diversity in response polarity, contrast sensitivity and temporal frequency tuning.
ReaChR-evoked responses had equivalent sensitivity for ON bipolar cell targeting and retinal ganglion cell targeting and could encode contrast across different background irradiances.
Quoted textsource-backed
For both targeting strategies, we find ReaChR-evoked responses have equivalent sensitivity and can encode contrast across different background irradiances.
In visually intact retinas, retinal ganglion cell-targeted ReaChR expression disrupted visual feature selectivity of individual retinal ganglion cells.
Quoted textsource-backed
Recording ReaChR-driven responses in visually intact retinas confirmed that RGC-targeted ReaChR expression disrupts visual feature selectivity of individual RGCs.
Both ON bipolar cell targeting and retinal ganglion cell targeting restored visual responses with high fidelity, but ON bipolar cell targeting produced a richer visual code that more closely approached wildtype mice.
Quoted textsource-backed
Our data show that while both approaches restore visual responses with impressive fidelity, ON BC targeting produces a richer visual code better approaching that of wildtype mice.
LiGluR-MAG0(460) targeted to either retinal ganglion cells or ON-bipolar cells in rd1 mice reinstated innate light-avoidance behavior and enabled discrimination of different temporal light patterns in an associative learning task.
Quoted textsource-backed
LiGluR-MAG0(460) in either RGCs or ON-BCs of the rd1 mouse reinstated innate light-avoidance behavior and enabled mice to distinguish between different temporal patterns of light in an associative learning task.
In blind rd1 mouse retinal explants, LiGluR-MAG0(460) targeted to retinal ganglion cells produced robust and uniform light-evoked firing.
Quoted textsource-backed
In the blind rd1 mouse, multielectrode array recordings of retinal explants revealed robust and uniform light-evoked firing when LiGluR-MAG0(460) was targeted to RGCs
In a rod-cone dystrophy dog model of blindness, LiGluR-MAG0(460) in retinal ganglion cells restored robust light responses in retinal explants and intravitreal delivery of LiGluR and MAG0(460) was well tolerated in vivo.
Quoted textsource-backed
In the rod-cone dystrophy dog model of blindness, LiGluR-MAG0(460) in RGCs restored robust light responses to retinal explants and intravitreal delivery of LiGluR and MAG0(460) was well tolerated in vivo.