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Zfhx3Sci/+ mouse missense mutation model

Candidate: concept label1 source documents5 linked claims
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Aliases

Zfhx3Sci, Zfhx3Sci/+, Zfhx3 Sci mutation

Extracted Explainers

What the tool is doing

The paper uses Zfhx3Sci/+ mice as a genetic model to test how a missense mutation in Zfhx3 affects growth, metabolism, and hypothalamic gene expression.

Source 1DOIPubMed

Resources required

Use of this model requires mice harboring the Zfhx3Sci/+ allele and comparison to Zfhx3+/+ littermates.

Source 1DOIPubMed

What problem it solves

It provides an in vivo system to study how altered ZFHX3 function influences energy balance-related phenotypes.

Source 1DOIPubMed

What it does not solve

The abstract does not show that this model identifies the exact molecular mechanism or isolates cell-type-specific effects.

Source 1DOIPubMed

Alternatives

The abstract contrasts the mutant mice with Zfhx3+/+ littermate controls.

Source 1DOIPubMed

Evidence Snippets

We investigated metabolic parameters in mice harboring a missense mutation in Zfhx3 (Zfhx3Sci/+).
Evidence 1Source 1DOIPubMedprovenance

Supporting Sources

Linked Claims

Claim 1feeding effectsupports2023Source 1DOIPubMed

Zfhx3Sci/+ mice eat less than wildtype controls in proportion to body weight.

Quoted textsource-backed
In a second cohort of 9-20-week-old males and females, Zfhx3Sci/+ mice ate less than wildtype controls, in proportion to body weight.
Claim 2gene expression changesupports2023Source 1DOIPubMed

The Zfhx3Sci mutation is associated with increased somatostatin expression and decreased Ghrh, Ghr, Npy, and Gpr50 expression in hypothalamic regions including the arcuate nucleus.

Quoted textsource-backed
We detected increased expression of somatostatin and decreased expression of growth hormone-releasing hormone and growth hormone-receptor mRNAs in the arcuate nucleus (ARC). Similarly, ARC expression of orexigenic neuropeptide Y was decreased and ventricular ependymal expression of orphan G protein-coupled receptor Gpr50 was decreased.
Claim 3mechanistic interpretationsupports2023Source 1DOIPubMed

The paper concludes that the Zfhx3Sci mutation affects energy balance likely by altering expression of key arcuate nucleus neuropeptides that influence growth, food intake, and energy expenditure.

Quoted textsource-backed
We demonstrate for the first time an energy balance effect of the Zfhx3Sci mutation, likely by altering expression of key ARC neuropeptides to alter growth, food intake, and energy expenditure.
Claim 4metabolic phenotyping resultsupports2023Source 1DOIPubMed

In female mice aged 6 to 14 weeks, Zfhx3Sci/+ animals weigh less and have lower lean mass and energy expenditure, while fat mass does not differ from controls.

Quoted textsource-backed
In a third cohort of female-only Zfhx3Sci/+ and Zfhx3+/+ mice that underwent metabolic phenotyping from 6 to 14 weeks old, Zfhx3Sci/+ mice weighed less and had lower lean mass and energy expenditure, but fat mass did not differ.
Claim 5phenotype effectsupports2023Source 1DOIPubMed

Zfhx3Sci/+ mice show reduced growth and altered metabolic traits relative to wildtype littermates.

Quoted textsource-backed
One-year-old male and female Zfhx3Sci/+ mice weighed less, had shorter body length, lower fat mass, smaller mesenteric fat depots, and lower circulating insulin, leptin, and insulin-like growth factor-1 (IGF1) concentrations than Zfhx3+/+ littermates.