First-pass extracted concept

5' deletion RhVI1 promoter-uidA reporter constructs

Candidate: toolkit itemType: construct pattern1 source documents3 linked claims

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Aliases

5' deletions of the proximal promoter fused to the uidA reporter gene

Evidence Snippets

5' deletions of the proximal promoter fused to the uidA reporter gene were inserted into the rose cell genome

Evidence 1Source 1DOI PubMedprovenance

Supporting Sources

Source 1primary paper2014Plant and Cell PhysiologyDOI PubMed

1 evidence snippet 3 linked claims Claim 1 Claim 2 Claim 3

Linked Claims

Claim 1functional sufficiencysupports2014Source 1DOI PubMed

A 468 bp RhVI1 promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars, and gibberellins.

Quoted textsource-backed

Deletion analysis revealed that the 468 bp promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars and gibberellins.

Claim 2stimulus specific responsesupports2014Source 1DOI PubMed

The 468 bp RhVI1 promoter region confers sucrose- and fructose-responsive, but not glucose-responsive, activation in the dark.

Quoted textsource-backed

This region confers sucrose- and fructose-, but not glucose-, responsive activation in the dark.

Claim 3synergistic regulationsupports2014Source 1DOI PubMed

Sugar plus light and gibberellin plus light act synergistically to sharply up-regulate GUS activity under control of the 595 bp pRhVI1 region.

Quoted textsource-backed

We also demonstrate that sugar/light and gibberellin/light act synergistically to up-regulate β-glucuronidase (GUS) activity sharply under the control of the 595 bp pRhVI1 region.