First-pass extracted concept

Arabidopsis mesophyll protoplast transient gene expression system

Candidate: toolkit itemType: assay method1 source documents4 linked claims

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Aliases

transient gene expression system using Arabidopsis mesophyll protoplasts

Extracted Explainers

What the tool is doing

This system enables transient gene expression analysis in Arabidopsis mesophyll protoplasts for a wide range of cell-based experiments. The abstract presents it as a versatile platform for studying signaling pathways and cellular machineries.

Source 1DOI PubMed

Resources required

The protocol includes protoplast isolation, PEG-calcium transfection of plasmid DNA, and protoplast culture. Results are reported as obtainable within 2-24 h after the 6-8 h isolation and transfection procedures.

Source 1DOI PubMed

What problem it solves

It provides a rapid, physiological, and high-throughput plant cell system for exploratory and hypothesis-driven functional analysis. The abstract emphasizes cost-effective experimentation across multiple assay modalities.

Source 1DOI PubMed

Evidence Snippets

The transient gene expression system using Arabidopsis mesophyll protoplasts has proven an important and versatile tool for conducting cell-based experiments...

Evidence 1Source 1DOI PubMedprovenance

Supporting Sources

Source 1primary paper2007Nature ProtocolsDOI PubMed

1 evidence snippet 4 linked claims Claim 1 Claim 2 Claim 3

Linked Claims

Claim 1protocol compositionsupports2007Source 1DOI PubMed

The reported method includes protoplast isolation, PEG-calcium transfection of plasmid DNA, and protoplast culture.

Claim 2throughput costsupports2007Source 1DOI PubMed

The Arabidopsis mesophyll protoplast system has physiological responses and high-throughput capability that enable facile and cost-effective explorations and hypothesis-driven tests.

Claim 3timingsupports2007Source 1DOI PubMed

The protoplast isolation and DNA transfection procedures take 6-8 hours, and results can be obtained in 2-24 hours.

Claim 4utilitysupports2007Source 1DOI PubMed

The Arabidopsis mesophyll protoplast transient gene expression system is an important and versatile tool for cell-based experiments across multiple molecular analysis modalities.