We hypothesized that a photoactivatable adenylyl cyclase (PAC) can be employed to modulate cAMP in β-cells with light thereby enhancing insulin secretion. To that end, the PAC gene from Beggiatoa (bPAC) was delivered to β-cells.
First-pass extracted concept
Beggiatoa photoactivatable adenylyl cyclase
Candidate: toolkit itemType: protein domain1 source documents4 linked claims
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Aliases
bPAC, PAC, photoactivatable adenylyl cyclase
Evidence Snippets
Supporting Sources
Linked Claims
bPAC expression and activation increase cAMP and insulin secretion in murine islets and β-cell pseudoislets, with pseudoislets showing more pronounced light-triggered hormone secretion than β-cell monolayers.
Quoted textsource-backed
Furthermore, the expression and activation of bPAC increased cAMP and insulin secretion in murine islets and in β-cell pseudoislets, which displayed a more pronounced light-triggered hormone secretion compared to that of β-cell monolayers.
Light activation of Beggiatoa photoactivatable adenylyl cyclase in pancreatic β-cells increases intracellular cAMP and enhances insulin secretion.
Quoted textsource-backed
A cAMP increase was noted within 5 minutes of photostimulation and a significant drop at 12 minutes post-illumination. The concomitant augmented insulin secretion was comparable to that from β-cells treated with secretagogues.
Calcium channel blocking reduces the enhanced insulin response produced by bPAC activity.
Quoted textsource-backed
Calcium channel blocking curtailed the enhanced insulin response due to bPAC activity.
Repeated cycles of bPAC photoinduction produce greater insulin release without adverse effects on viability and proliferation.
Quoted textsource-backed
Greater insulin release was also observed over repeated cycles of photoinduction without adverse effects on viability and proliferation.