A photoactivatable CRISPR/Cas9 system consisting of the Cas9 protein, synthetic 102-nt sgRNA or a pair of guide crRNA/tracrRNA, and blocking photocleavable oligodeoxyribonucleotides has been designed.
First-pass extracted concept
blocking photocleavable oligodeoxyribonucleotides
Candidate: toolkit item1 source documents3 linked claims
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Aliases
photomodified blocking oligodeoxyribonucleotides
Evidence Snippets
Supporting Sources
Linked Claims
A photoactivatable CRISPR/Cas9 system composed of Cas9, guide RNA, and blocking photocleavable oligodeoxyribonucleotides was designed.
Quoted textsource-backed
A photoactivatable CRISPR/Cas9 system consisting of the Cas9 protein, synthetic 102-nt sgRNA or a pair of guide crRNA/tracrRNA, and blocking photocleavable oligodeoxyribonucleotides has been designed.
UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can produce photoactivatable gene editing in vitro.
Quoted textsource-backed
It has been shown that UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can result in photoactivatable gene editing in vitro.
The thermal stability of complexes between modified blocking oligodeoxyribonucleotides and guide crRNA decreases as the number of photodegradable linkers increases.
Quoted textsource-backed
The thermal stability of complexes of these modified oligonucleotides with guide crRNA decreases with increasing the number of linkers.