Three light-gated channels, ChR2-XXM 2.0 (light-gated cation channel), GtACR1 (light-gated anion channel) and SthK-bPAC (light-gated potassium channel), were used in my study to regulate ion transport by light and thus manipulate the osmotic gradient and water transport.
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ChR2-XXM 2.0
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ChR2-XXM 2.0, GtACR1, and SthK-bPAC were used to regulate ion transport by light and thereby manipulate osmotic gradient and water transport.
Quoted textsource-backed
Three light-gated channels, ChR2-XXM 2.0 (light-gated cation channel), GtACR1 (light-gated anion channel) and SthK-bPAC (light-gated potassium channel), were used in my study to regulate ion transport by light and thus manipulate the osmotic gradient and water transport.
Coexpression of ChR2-XXM 2.0, GtACR1, and AQP1 in Xenopus oocytes under blue light triggers Na+ influx, facilitates Cl- influx, forms an osmotic gradient, and drives water influx through AQP1.
Quoted textsource-backed
When expressing ChR2-XXM 2.0 and GtACR1 together, mainly Na+ influx was triggered by ChR2-XXM2.0 under blue light illumination, which then made the membrane potential more positive and facilitated Cl- influx by GtACR1. Due to this inward movement of Na+ and Cl-, the osmotic gradient was formed to trigger water influx through AQP1.
Large water uptake in the ChR2-XXM 2.0/GtACR1/AQP1 Xenopus oocyte system can rapidly increase oocyte volume until membrane rupture.
Quoted textsource-backed
Large amounts of water uptake can speedily increase the oocyte volume until membrane rupture.