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CHS promoter-GUS fusion constructs

Candidate: toolkit itemType: construct pattern1 source documents5 linked claims
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Aliases

fusion constructs between CHS promoters from parsley or mustard and the β-glucuronidase reporter gene (GUS)

Evidence Snippets

observations on the photoregulation of fusion constructs between CHS promoters from parsley or mustard and the β‐glucuronidase reporter gene ( GUS )
Evidence 1Source 1DOIprovenance

Supporting Sources

Linked Claims

Claim 1assay recapitulationsupports1992Source 1DOI

In the parsley protoplast transient expression system, both CHS promoter-GUS constructs yielded the same type of photoregulation as the endogenous CHS gene.

Quoted textsource-backed
When tested in the parsley protoplast transient expression system, both constructs yielded the same type of photoregulation as observed for the endogenous CHS gene.
Claim 2mechanistic exclusionsupports1992Source 1DOI

Reduced red light control in mature leaves was not due to absence of immunoreactive phytochrome.

Quoted textsource-backed
The reduced red light control in mature leaves was not due to the absence of immunoreactive phytochrome.
Claim 3photoregulation observationsupports1992Source 1DOI

In leaves of adult re-etiolated plants, a UV-B photoreceptor was predominantly involved in photocontrol of CHS expression.

Quoted textsource-backed
In leaves of adult re‐etiolated plants, a UV‐B photoreceptor was predominantly involved in photocontrol.
Claim 4photoregulation observationsupports1992Source 1DOI

In parsley and mustard, CHS mRNA accumulation in young etiolated seedlings was primarily under phytochrome control.

Quoted textsource-backed
In both species, CHS mRNA accumulation in young etiolated seedlings was primarily under phytochrome control.
Claim 5reporter construct agreementsupports1992Source 1DOI

Photoreceptor usage depended on developmental state, and this pattern was consistent with observations from parsley or mustard CHS promoter-GUS fusion constructs.

Quoted textsource-backed
The apparent dependence of photoreceptor usage on the developmental state of the cell or organism was in accordance with observations on the photoregulation of fusion constructs between CHS promoters from parsley or mustard and the β‐glucuronidase reporter gene ( GUS ).