First-pass extracted concept

CRY2-CIB1 optogenetic system

Candidate: toolkit itemType: multi component switch3 source documents5 linked claims

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Aliases

CRY2-CIB1

Evidence Snippets

Here, we use the CRY2-CIB1 optogenetic system to recruit and cluster a cytosolic Cdc42 variant at the plasma membrane

Evidence 1Source 1DOI PubMedprovenance

Here, we use the CRY2-CIB1 optogenetic system to recruit and cluster a cytosolic Cdc42 allele at the plasma membrane

Evidence 2Source 2DOIprovenance

We implemented the CRY2-CIB1 optogenetic system for acute light-dependent protein recruitment to the plasma membrane

Evidence 3Source 3DOI PubMedprovenance

Supporting Sources

Source 1primary paper2020CellsDOI PubMed

1 evidence snippet 1 linked claim Claim 1

Source 2primary paper2020DOI

1 evidence snippet 1 linked claim Claim 1

Source 3primary paper2020PLoS BiologyDOI PubMed

1 evidence snippet 3 linked claims Claim 1 Claim 2 Claim 3

Linked Claims

Claim 1conclusionsupports2020Source 3DOI PubMed

Scaffold-mediated positive feedback gated by Ras activity confers robust polarization for rod-shape formation.

Quoted textsource-backed

We conclude that scaffold-mediated positive feedback, gated by Ras activity, confers robust polarization for rod-shape formation.

Claim 2implementationsupports2020Source 3DOI PubMed

The study implemented the CRY2-CIB1 optogenetic system for acute light-dependent protein recruitment to the plasma membrane in Schizosaccharomyces pombe.

Quoted textsource-backed

We implemented the CRY2-CIB1 optogenetic system for acute light-dependent protein recruitment to the plasma membrane

Claim 3mechanismsupports2020Source 3DOI PubMed

Optogenetic recruitment of constitutively active Cdc42 leads to co-recruitment of Scd1 and endogenous Cdc42 in a Scd2-dependent manner.

Quoted textsource-backed

optogenetic recruitment of constitutively active Cdc42 leads to co-recruitment of the guanine nucleotide exchange factor (GEF) Scd1 and endogenous Cdc42, in a manner dependent on the scaffold protein Scd2

Claim 4tool effectsupports2020Source 2DOI

The CRY2-CIB1 optogenetic system was used to recruit and cluster a cytosolic Cdc42 allele at the plasma membrane, leading to moderate activation on cell sides.

Quoted textsource-backed

Here, we use the CRY2-CIB1 optogenetic system to recruit and cluster a cytosolic Cdc42 allele at the plasma membrane and show that this leads to its moderate activation also on cell sides.

Claim 5tool effectsupports2020Source 1locatorDOI PubMed

Using the CRY2-CIB1 optogenetic system to recruit and cluster a cytosolic Cdc42 variant at the plasma membrane leads to moderate Cdc42 activation on cell sides.

Quoted textsource-backed

Here, we use the CRY2-CIB1 optogenetic system to recruit and cluster a cytosolic Cdc42 variant at the plasma membrane and show that this leads to its moderate activation also on cell sides.