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Evol-GATE strain

Candidate: toolkit itemType: construct pattern1 source documents5 linked claims
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Extracted Explainers

What the tool is doing

Evol-GATE is the post-selection yeast strain isolated after growth-coupled evolution and plasmid curing. In the abstract it is the final strain showing increased intracellular heme and pathway upregulation.

Source 1DOIPubMed

Resources required

The abstract indicates that generating this strain required continuous culture under growth-selective pressure followed by plasmid curing and genomic analysis.

Source 1DOIPubMed

What problem it solves

It addresses the need for a yeast strain with higher heme content for single-cell protein and meat-analogue applications.

Source 1DOIPubMed

What it does not solve

The abstract does not establish detailed production performance beyond intracellular heme increase and notes a slight biomass reduction.

Source 1DOIPubMed

Alternatives

The parental type is the direct comparator mentioned in the abstract; the broader alternative strategy implied is rational metabolic engineering rather than growth-coupled evolution.

Source 1DOIPubMed

Evidence Snippets

After 100 h of continuous culture under growth-selective pressure, we cured out the plasmid to isolate an Evol-GATE strain.
Evidence 1Source 1DOIPubMedprovenance

Supporting Sources

Linked Claims

Claim 1application relevancesupports2026Source 1DOIPubMed

GATE can effectively select yeast mutants with significantly improved heme productivity for development of non-GMO single-cell proteins enriched in heme.

Claim 2genomic characterizationsupports2026Source 1DOIPubMed

Whole-genome sequencing found only a small number of dispersed variants and no residual plasmid sequences in Evol-GATE, supporting classification as a non-GMO mutant.

Claim 3performance improvementsupports2026Source 1DOIPubMed

The Evol-GATE strain showed a five-fold increase in intracellular heme compared with the parental type.

Claim 4tradeoffsupports2026Source 1DOIPubMed

The Evol-GATE strain had a slight reduction in biomass relative to the parental type.

Claim 5transcriptomic changesupports2026Source 1DOIPubMed

Transcriptome analysis confirmed increased expression of heme biosynthesis and associated respiratory genes in Evol-GATE.