First-pass extracted concept

homing endonuclease

Candidate: toolkit item1 source documents4 linked claims

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Aliases

HEase

Evidence Snippets

expression of the homing endonuclease (HEase)

Evidence 1Source 1DOI PubMedprovenance

Supporting Sources

Source 1primary paper2016PLoS ONEDOI PubMed

1 evidence snippet 4 linked claims Claim 1 Claim 2 Claim 3

Linked Claims

Claim 1potential applicationsupports2016Source 1DOI PubMed

Attenuating homing endonuclease activity might be useful for precision genome engineering to minimize off-target activities or alter pathways during a specific growth phase.

Quoted textsource-backed

Ultimately the ability to attenuate HEase activity might be useful in precision genome engineering, minimizing off target activities, or where pathways have to be altered during a specific growth phase.

Claim 2regulates expressionsupports2016Source 1DOI PubMed

Manipulating the splicing efficiency of HEase ORF-embedded group II introns can regulate homing endonuclease expression in vivo in Escherichia coli.

Quoted textsource-backed

This study showed that the expression of the HEase (in vivo) in Escherichia coli can be regulated by manipulating the splicing efficiency of the HEase ORF-embedded group II introns.

Claim 3small molecule modulationsupports2016Source 1DOI PubMed

Cobalt chloride added to growth media antagonized homing endonuclease activity.

Quoted textsource-backed

the addition of cobalt chloride (CoCl2) to growth media antagonized the expression of HEase activity

Claim 4small molecule modulationsupports2016Source 1DOI PubMed

Exogenous magnesium chloride stimulated expression of a functional homing endonuclease.

Quoted textsource-backed

Exogenous magnesium chloride (MgCl2) stimulated the expression of a functional HEase