First-pass extracted concept

iLight9O

Candidate: toolkit itemType: multi component switch1 source documents3 linked claims

Jump to explainers Jump to evidence Jump to sources Jump to claims

Live refresh every 5sNext refresh in 5s

Extracted Explainers

What the tool is doing

iLight9O is a stabilized blue light-inducible optogenetic CRISPR-dCas9 system that enables programmable regulation of distinct genes using specific sgRNAs. In this paper it is used for dynamic repression during patchoulol pathway engineering.

Source 1DOI PubMed

Resources required

The system requires sgRNAs and the underlying CRISPR-dCas9 platform, and its improved version includes a protein degradation tag strategy. The abstract does not specify the exact tag or architecture.

Source 1DOI PubMed

What problem it solves

It addresses stability limitations of iLight9 and supports dynamic control of endogenous gene expression without the promoter target-site insertion constraint described for prior systems.

Source 1DOI PubMed

What it does not solve

The abstract does not show that iLight9O alone is sufficient for high patchoulol production without additional pathway engineering such as IU pathway introduction.

Source 1DOI PubMed

Alternatives

The source contrasts iLight9O with static engineering approaches and with earlier yeast optogenetic repression systems that require target-site insertion near native promoters.

Source 1DOI PubMed

Evidence Snippets

The stability of the iLight9 system was further enhanced by employing a strategy involving the addition of a protein degradation tag. The resulting system was designated as iLight9O, which facilitated programmable regulation of distinct genes through the introduction of specific sgRNAs.

Evidence 1Source 1DOI PubMedprovenance

Supporting Sources

Source 1primary paper2026MEDDOI PubMed

1 evidence snippet 3 linked claims Claim 1 Claim 2 Claim 3

Linked Claims

Claim 1application performancesupports2026Source 1DOI PubMed

Using iLight9O for dynamic downregulation of squalene synthase increased patchoulol titers by 66% in the IU-optimized strain and 24% in the MVAIU2 strain compared with static engineering approaches.

Quoted textsource-backed

the iLight9O system was adopted to dynamically downregulate squalene synthase, a key enzyme in the competing squalene biosynthetic pathway. This optogenetic flux control strategy increased patchoulol titers by 66 % in the IU-optimized strain and 24 % in the MVAIU2 strain, demonstrating significant improvements over static engineering approaches.

Claim 2tool capabilitysupports2026Source 1DOI PubMed

iLight9O facilitates programmable regulation of distinct genes through specific sgRNAs.

Quoted textsource-backed

iLight9O, which facilitated programmable regulation of distinct genes through the introduction of specific sgRNAs

Claim 3tool improvementsupports2026Source 1DOI PubMed

Adding a protein degradation tag enhanced the stability of iLight9 and produced the resulting system iLight9O.

Quoted textsource-backed

The stability of the iLight9 system was further enhanced by employing a strategy involving the addition of a protein degradation tag. The resulting system was designated as iLight9O