First-pass extracted concept

in vitro assembled Cas9-ribonucleoprotein complexes

Candidate: toolkit item1 source documents3 linked claims

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Aliases

Cas9-ribonucleoprotein complexes, RNPs

Evidence Snippets

using in vitro assembled RNPs

Evidence 1Source 1DOI PubMedprovenance

Supporting Sources

Source 1primary paper2018Fungal Biology and BiotechnologyDOI PubMed

1 evidence snippet 3 linked claims Claim 1 Claim 2 Claim 3

Linked Claims

Claim 1efficiencysupports2018Source 1DOI PubMed

The in vitro assembled Cas9-ribonucleoprotein and microhomology template system is highly efficient.

Quoted textsource-backed

This system is highly efficient

Claim 2off target specificitysupports2018Source 1DOI PubMed

The system is not associated with increased off-target mutations caused by introduction of the Cas9 nuclease.

Quoted textsource-backed

is not associated with increased off-target mutations caused by introduction of the Cas9 nuclease

Claim 3reliabilitysupports2018Source 1DOI PubMed

CRISPR/Cas9-based genome editing in Aspergillus fumigatus using in vitro assembled Cas9-ribonucleoprotein complexes coupled with microhomology templates is a reliable method of gene targeting.

Quoted textsource-backed

CRISPR/Cas9-based genome editing in <i>A. fumigatus</i> using in vitro assembled RNPs coupled with microhomology templates is a reliable method of gene targeting.