Light-activated reversible inhibition by assembled trap (LARIAT) relies on the rapid light-dependent heterodimerization between cryptochrome 2 (CRY2) and cryptochrome-interacting bHLH 1 (CIB1) to form large protein clusters.
First-pass extracted concept
light-activated reversible inhibition by assembled trap
Candidate: toolkit item1 source documents6 linked claims
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Aliases
GFP-based LARIAT, GFP-LARIAT, LARIAT
Evidence Snippets
Supporting Sources
Linked Claims
LARIAT was adapted for use in Drosophila S2 cells.
Quoted textsource-backed
In this research study, we report the adaptation of an optogenetic tool to <i>Drosophila</i> S2 cells.
GFP-based LARIAT enables probing of dynamic processes and protein-protein interactions with high spatiotemporal resolution in Drosophila S2 cells.
Quoted textsource-backed
GFP-based LARIAT is a versatile tool to answer different biological questions, since it enables probing of dynamic processes and protein-protein interactions with high spatiotemporal resolution in <i>Drosophila</i> S2 cells.
GFP-LARIAT can disrupt membrane localization of the polarity regulator Lgl in Drosophila S2 cells.
Quoted textsource-backed
and to disrupt the membrane localization of the polarity regulator Lethal Giant Larvae (Lgl)
GFP-LARIAT can inactivate the mitotic protein Mps1 in Drosophila S2 cells.
Quoted textsource-backed
we evaluated clustering kinetics in response to light for different LARIAT modules, and showed the ability of GFP-LARIAT to inactivate the mitotic protein Mps1
An anti-GFP nanobody fused with CRY2 enables LARIAT to trap GFP-tagged proteins in light-induced protein clusters.
Quoted textsource-backed
An anti-green fluorescent protein (GFP) nanobody fused with CRY2 allows this method to quickly trap any GFP-tagged protein in these light-induced protein clusters.
LARIAT uses rapid light-dependent CRY2-CIB1 heterodimerization to form large protein clusters.
Quoted textsource-backed
Light-activated reversible inhibition by assembled trap (LARIAT) relies on the rapid light-dependent heterodimerization between cryptochrome 2 (CRY2) and cryptochrome-interacting bHLH 1 (CIB1) to form large protein clusters.