First-pass extracted concept

LOV2-AraC optogenetic switch

Candidate: toolkit itemType: multi component switch1 source documents3 linked claims

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Aliases

AraC optogenetic switches

Evidence Snippets

Applying this framework to the transcription factor AraC yielded near-binary optogenetic switches

Evidence 1Source 1DOIprovenance

Supporting Sources

Source 1primary paper2025DOI

1 evidence snippet 3 linked claims Claim 1 Claim 2 Claim 3

Linked Claims

Claim 1mechanismsupports2025Source 1DOI

Linker mutations that promote alpha-helix extension at the sensor-effector junction enhance conformational coupling between LOV2 and AraC.

Quoted textsource-backed

Mechanistically, we found that linker mutations promoting α-helix extension at the sensor-effector junction enhance conformational coupling between LOV2 and AraC.

Claim 2performancesupports2025Source 1DOI

Applying the framework to AraC yielded near-binary optogenetic switches with light-controlled activity spanning approximately 1,000-fold dynamic range.

Quoted textsource-backed

Applying this framework to the transcription factor AraC yielded near-binary optogenetic switches, with light-controlled activity spanning ∼1,000-fold dynamic range.

Claim 3reproducibility signalsupports2025Source 1DOI

Variants with the identified linker mutations emerged consistently across independently evolved pools.

Quoted textsource-backed

These variants emerged consistently across independently evolved pools, underscoring their functional relevance.